electrophoresis

(redirected from Two-dimensional gel electrophoresis)
Also found in: Dictionary, Thesaurus, Acronyms, Encyclopedia, Wikipedia.

electrophoresis

 [e-lek″tro-fo-re´sis]
the movement of charged particles suspended in a liquid on various media (e.g., paper, gel, liquid) under the influence of an applied electric field. adj., adj electrophoret´ic. The various charged particles of a particular substance migrate in a definite and characteristic direction—toward either the anode or the cathode—and at a characteristic speed. This principle has been widely used in the separation of proteins and is therefore valuable in the study of diseases in which the serum and plasma proteins are altered. The principle also has been applied in the separation and identification of various types of human hemoglobin.

e·lec·tro·pho·re·sis

(ē-lek'trō-fōr'ē-sis),
The movement of particles in an electric field toward an electric pole (anode or cathode); used to separate and purify biomolecules.
See also: electropherogram.
[electro- + G. phorēsis, a carrying]

electrophoresis

(ĭ-lĕk′trō-fə-rē′sĭs)
n.
1. The migration of charged colloidal particles or molecules through a stationary medium under the influence of an applied electric field usually provided by immersed electrodes. Also called cataphoresis.
2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.

e·lec′tro·pho·ret′ic (-rĕt′ĭk) adj.

electrophoresis

Lab methods A method of separating large molecules–eg, DNA fragments or proteins from a mixture of similar molecules, by passing an electric current through a medium containing the mixture; each molecule travels through the medium at a different rate, depending on its electrical charge and size; agarose and acrylamide gels are media commonly used electrophoretic media

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
See also: electropherogram
Synonym(s): ionophoresis, phoresis (1) .

electrophoresis

Separation of charged particles in a solution (ions) by the application of an electric current. This can be done in a thin layer of solution on paper or in a gel. Ions of low weight move more quickly than those of high weight, so separation occurs and can be demonstrated by staining. The method is widely used in medicine to identify and measure the proteins present in the blood including the ANTIBODIES (IMMUNOGLOBULINS). It is used to identify the various abnormal haemoglobins causing SICKLE CELL ANAEMIA and other similar conditions. It is extensively used in genetic work such as DNA fingerprinting. Electrophoresis is remarkably sensitive. Pieces of DNA, for instance, that differ in length from each other by only one base pair can be separated into discrete bands by this method.

electrophoresis

a method for separating particles with different electrical charges, for example, amino acids, peptides, proteins and nucleic acids. The apparatus consists of a supporting medium soaked in a suitable buffer with an electrical field set up across it. The mixture to be separated (e.g. blood proteins) is placed on the supporting medium. The components with different charges then separate from each other and their eventual position is compared with the position of known standards.

Electrophoresis

Use of an electrical field to separate proteins in a mixture (such as blood or urine), on the basis of the size and electrical charge of the proteins.

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
Synonym(s): ionophoresis, phoresis (1) .
References in periodicals archive ?
The patterns of synthesis of individual proteins were compared, using two-dimensional gel electrophoresis [ILLUSTRATION FOR FIGURES 7, 8 OMITTED], for the trochophore stage (1-day-old) and a veliger stage (3-day-old).
Nevertheless, to our knowledge, the work presented here is the first to identify a biomarker for metastasis based on a combination of SELDI-TOF M5, two-dimensional gel electrophoresis, in-gel trypsin digestion, PMF, and MS/MS followed by validation by ELISA and immunodepletion assay, with the results obtained with the various approaches compared with results obtained with machine-learning algorithms.
Two-dimensional gel electrophoresis (2-DE) was performed on both HF and HC groups with the method as previously described by Chen et al.
Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis. Proteome Sci.
The first major breakthrough, which was a great leap forward in separation, took place in 1975 with the introduction of two-dimensional gel electrophoresis (2DE).
Eight human pituitary control tissues were used to extract proteins, and each control tissue was analyzed (three to five times) by two-dimensional gel electrophoresis (2DGE).
For example, two-dimensional gel electrophoresis and MS/MS are being used for global protein separation and protein identification.
The aim of this study was to use a serologic proteomic analysis, including one- and two-dimensional gel electrophoresis, immunoblot analysis, and matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) for the reliable characterization and identification of putative targets of anti-SLA antibodies.
Two-dimensional gel electrophoresis has been the mainstay of electrophoretic technology for a decade and is the most widely used tool for separating proteins.
The second part describes 15 selected methods, including two-dimensional gel electrophoresis. Capillary electrophoresis is not included.

Full browser ?