The study used RNA sequencing (RNA-Seq), two-dimensional electrophoresis
(2-DE), and mass spectrometry (MS) to investigate the transcriptome and proteome of the MDR isolate and a control strain of C.
based proteomic analysis of the pea (Pisum sativum) in response to Mycosphaerella pinodes.
(2013) optimized the methods of two-dimensional electrophoresis
system of mulberry fruits, and Niu et al.
An inappropriate labeling of several fish species, commercially sold under the general label "perch," was revealed using Isoelectric Focusing (IEF) and two-dimensional electrophoresis
(2-DE) for the correct fish identification .
For two-dimensional electrophoresis
(2DE) purposes, a rehydration buffer [8 M urea, 1% (m/v) CHAPS, 40 mM DTT, 0.25% (v/v) Biolytes 3-10] was added to the liver extract (100-400 [micro]g), avoiding to exceed 150 [micro]L of deposited sample.
Methods: Samples of the uterosacral ligament (USL) were collected from five POP patients and five non-POP patients matched according to age, parity, and menopausal status and analyzed using two-dimensional electrophoresis
and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
High resolution two-dimensional electrophoresis
of basic as well as acidic proteins.
. 1 x [10.sup.7] BLZ211 cells were suspended in 200 [micro]L of lysis buffer (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% Triton X-100, 0.5% IPG buffer, 2mM TBP, and 50 mM DTT) supplemented with protease inhibitor cocktail and vortexed at 4[degrees]C for 1h, and then the cell lysate was clarified by centrifugation at 40,000 xg for 1 h.
SDS-PAGE two-dimensional electrophoresis
was repeated for three times.
From 2004 to 2006, we performed pH 3-10 and pH 4-7 gradient two-dimensional electrophoresis
(2-DE) on tissue samples of nasal polyps, chronic sinusitis, and normal nasal mucosa.
During the last two decades, two-dimensional electrophoresis
(2-DE) gel has established itself as the de facto approach to separating proteins from cell and tissue samples.
of myocardial proteins in human cardiovascular diseases.