For two-dimensional electrophoresis
(2DE) purposes, a rehydration buffer [8 M urea, 1% (m/v) CHAPS, 40 mM DTT, 0.
Methods: Samples of the uterosacral ligament (USL) were collected from five POP patients and five non-POP patients matched according to age, parity, and menopausal status and analyzed using two-dimensional electrophoresis
and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
The aim of this study was to establish and optimize the two-dimensional electrophoresis
(2-DE) technology for hydatid fluid proteome of the Echinococcus granulosus and obtain 2-DE map of hydatid fluid proteome.
High resolution two-dimensional electrophoresis
of basic as well as acidic proteins.
This research aimed at establishment of a two-dimensional electrophoresis
that is suitable for the proteomic analysis of ramie's various parts by optimizing the traditional TCA/acetone protein extraction method: protein purification (4 times volume of acetone), and IPG strips (pH 4-7, 17 cm).
From 2004 to 2006, we performed pH 3-10 and pH 4-7 gradient two-dimensional electrophoresis
(2-DE) on tissue samples of nasal polyps, chronic sinusitis, and normal nasal mucosa.
During the last two decades, two-dimensional electrophoresis
(2-DE) gel has established itself as the de facto approach to separating proteins from cell and tissue samples.
of myocardial proteins in human cardiovascular diseases.
was carried out according to the manufacturer's instmctions.
(2DE) is a popular technique for proteomic comparisons, which was recently revolutionized by the development of immobilized pH gradient strips for reproducible separations of matched samples of proteins encoded on genes.
Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis
Proteomics involves the analysis of all proteins expressed in a genome and uses a combination of sophisticated technologies such as two-dimensional electrophoresis
, mass spectrometry and bioinformatics to identify and characterize proteins.