two-dimensional chromatography

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two-di·men·sion·al chro·ma·tog·ra·phy

paper chromatography in which a spot, located originally in one corner of a sheet, is developed in one direction along one side of the sheet, after which the sheet is rotated 90° and developed, with another solvent, in the new direction; the resultant spots are thus spread over the entire paper, producing a "map" or "fingerprint." Also generalized to include chromatography followed by electrophoresis (or vice versa) and column chromatography followed by paper chromatography.
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* Two-dimensional chromatography of amniotic fluid phospholipids is the gold standard for prenatal assessment of fetal pulmonary maturity.

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