Each biochemical test was done with known controls.11 Indole Production (IP) test was done in which bacteria split amino acid tryptophan into indole and pyruvic acid using the enzyme tryptophanase
. Ehrlich reagent containing an aldehyde was used to detect indole production.
Indole catabolism is supported by tryptophanase
, which can be induced by tryptophan or suppressed by glucose.
Snell, "Formation and interrelationships of tryptophanase
and tryptophan synthetases in Escherichia coli," Journal of Bacteriology, vol.
coli by disrupting indole signaling, which is increased as a result of high tryptophanase
was also significantly downregulated in male animals but not in females (Figure 3D).
pneumoniae which show negative reaction because they are non motile and can not produce tryptophanase
enzyme as well as does not reduce sodium thiosulphate in the medium (Table 1).
A colorimetric method for the determination of indole, and its application to assay of tryptophanase
. Anal Biochem 1978;86:457-462.
The essential amino acid tryptophan has been hypothesized to be the ultimate precursor for indole production, in which tryptophanase
enzymes act on tryptophan to produce indoles (Verhecken 1989, Naegel & Alvarez 2005, Westley et al.
The production of tryptophanase
, sufficient amount of alcohol, acetyl methyl carbinol, and citrate utilisation were noted using the indole test, methyl red (MR) test, Voges-Proskauer (VP) test, and citrate utilization test, respectively, according to the protocol of Mackie McCartney .
* tryptophan for detection of tryptophanase
activity (indole production) and of tryptophan deaminase activity.
operon (tnaOP) is a region consisting of two major structural genes: tnaA, coding for the tryptophanase
which catalyzes the degradation of L-tryptophan to indole, pyruvate, and ammonia and tnaB coding for a tryptophan permease.
Also, it was catalase, oxidase, caseinase, and tryptophanase