Moreover, it was clear that kirk
tryptone media resulted in the highest laccase production which reached over 900 U/ng protein at 6 days of incubation as compared to over 500 U/ng protein in only two days, this is higher than the activity resulted from incubation with other media.
Bacteria were subcultured from glycerol stored cultures on
tryptone soy agar medium overnight and DNA was extracted.
Suitable amino acid sources include peptone,
tryptone, soytone, yeast extract and beef extract.
Initial enrichment was made in
Tryptone soy broth (TSB) (Hi-Media, Mumbai, India) supplemented with 500U/ ml of polymyxin B at 37[degrees]C for 24-48 h followed by streaking on B.
Citrate phosphate buffer (CPB), Phosphate buffered saline (PBS), Glutarldehyde, Isopropanol, Malachite green stain, Acridine orange stain (Himedia), Peptone water, Nutrient broth (NB) (Himedia, India), Nutrient agar (NA) (Himedia),
Tryptone soya broth (TSB) (Himedia), Mueller Hinton Agar (MHA) (Himedia).
9P were routinely grown in
Tryptone Soy Broth (Oxoid) supplemented with 0.
In the nutrien rich
tryptone soy broth (TSB), all strains produced more biofilm than in the nutrient poor
tryptone broth (TB).
Hodson et al (7) used a broth microdilution method with
tryptone soya broth and found S.
5[degrees] for 24 hours and (ii) a Simmons citrate agar slant and a tube of
tryptone broth (for the indole test), which were both incubated at 35[degrees]C for 48 hours.
coli (lauryl
tryptone broth with inverted Durham tubes) were inoculated (1 mL in 8 mL media).
After overnight incubation at 26 [degrees]C on 2xYT (16 g/L
tryptone, 10 g/L yeast extract, 5 g/L NaCI) agar plates containing 10 g/L glucose and 25 mg/L chloramphenicol, phages displaying scFv were prepared for panning by rescue using the M13K07 helper phage.
coli cells were incubated at 37[degrees]C for 1 hr at 225 rpm with additional 250 [micro]L SOC medium (2%
tryptone, 0.