tryptone


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tryp·tone

(trip'tōn),
A peptone produced by proteolytic digestion with trypsin.
Farlex Partner Medical Dictionary © Farlex 2012

tryptone

(trĭp′tōn)
A peptide produced by the action of trypsin on a protein.
Medical Dictionary, © 2009 Farlex and Partners
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gilamemis BSH-3T Nitrogen source Enzyme activity (IU) Cell mass (mg/ml) Beef extract 160.8[+ or -]3.4 0.82[+ or -]0.013 Malt extract 152.4[+ or -]4.2 0.76[+ or -]0.023 Peptone 158.2[+ or -]5.2 0.78[+ or -]0.031 Tryptone 156.4[+ or -]6.7 0.74[+ or -]0.035 Urea 123.2[+ or -]3.4 0.43[+ or -]0.024 Yeast extract 220.2[+ or -]5.2 1.40[+ or -]0.034 Table 4.
All the diluted samples were cultured on two plates of different culture media, inorganic salts starch agar (ISP4) media supplemented with cycloheximide (100pg/ml) and colistin (5pg/ ml) for Actinomycetes; and tryptone soya agar supplemented with cycloheximide (30pg/ml) and penicillin (100pg/ml) for Eubacteria.
For the determination of total aerobic mesophilic bacteria, dilutions of the water samples were made in tryptone salt and then 1 mL of each dilution was incorporated in plate count agar medium (Biokar Diagnostics) and incubated at 30[degrees]C for 72 h.
The media, including Luria Bertani broth (LB) (tryptone, 10.0 g/L; yeast extract, 5.0 g/L; NaCl, 10.0 g/L), tryptone soy broth (TSB) (tryptone, 17.0 g/L; soytone, 3.0 g/L; glucose, 2.5 g/L; NaCl, 5.0 g/L; [K.sub.2]HP[O.sub.4], 2.5 g/L), and modified yeast extract broth (YSG) (glycerol, 5.0 mL/L; yeast extract, 5.0 g/L; 1 M MgS[O.sub.4], 5.0 mL/L; [(N[H.sub.4]).sub.2]S[O.sub.4], 2.0 g/L; 1 M K[H.sub.2]O[P.sub.4], 5.0 mL/L; 1 M [K.sub.2]HO[P.sub.4], 5.0 mL/L; and 1 M [Na.sub.2]S[O.sub.4], 10.0 mL/L) were used in comparative studies to find the optimal nutrient medium for maximizing the antifungal activity.
Cultures with these confirmed nonpathogenic or pathogenic strains were stored at -80[degrees]C in modified tryptone soy broth (mTSB) (Land Bridge Technology Co., Ltd., Beijing, China) supplemented with 3% NaCl and adjusted to pH 8.0 [+ or -] 0.2) with addition of 25% (v/v) glycerol (National Pharmaceutical Group Corporation, Shanghai, China).
Antimicrobial assay: Before inoculation, all Staphylococcus spp isolates were transferred from the stock cultures to Tryptone Soya Agar (TSA) (Oxoid-CM0131) and incubated overnight at 37 AdegC.
For experimental use, freeze-dried cultures of the organisms were grown on tryptone soya agar (Oxoid) at 37[degrees]C.
The plate was incubated overnight at 4 [degrees]C and subsequently washed once with 0.05% PBST, and blocking solution (1% tryptone, 1% BSA, 3% sucrose (150 [micro]L/well)) in PBS for 1 h at 37 [degrees]C was then added.
Axenic cultures of Tetrahymena pyriformis were obtained from Carolina Biological Supply Company (Carolina) and maintained in Carolina's growth medium (proteose peptone, 5 g/l; tryptone, 5 g/l; [K.sub.2]HP[O.sub.4], 1.1 mmol/l).
sullae were grown on the yeast extract mannitol agar (YMA) solid medium (mannitol, 10 g/liter; [KH.sub.2][PO.sub.4], 0.5 g/liter; [MgSO.sub.4].7[H.sub.2]O, 0.2 g/liter; NaCl, 0.1 g/liter; yeast extract, 0.5 g/liter; agar, 15 g/liter), on the Tryptone-yeast (TY) broth medium (Tryptone, 5 g/liter; yeast extract, 3 g/liter; [CaCl.sub.2].[H.sub.2]O, 0.87g/liter), or Tryptone- yeast agar (TYA) solid medium (TY supplemented with 12g of agar in liter) at 28[degrees]C for 24h.