For the quantification of
tryptic digestibility the incubated mixtures of Amaranth bound proteins (BSA and Nisin) were digested separately by trypsin (at an enzyme concentration of 1 mg/50 mg of substrate) at various time intervals [16].
Quantification of serum IgG subclasses by use of subclass-specific
tryptic peptides and liquid chromatography-tandem mass spectrometry.
ruckeri: Inoculations were made from peritoneal fluid, stomach content, liver, kidney and blood samples of the fish and aquarium water onto
Tryptic Soy Agar (TSA) (Difco) and Shotts- Waltman (SW) (Oxoid) culture media and the cultures were incubated at 24 oC for two days.
The assay presented here that uses subclass-specific
tryptic peptides coupled with LC-MS/MS eliminates inconsistencies observed with immunoassays.
Agar strip-
Tryptic soy agar (TSA) strip for total bacterial count in air, Albert stain, Alkaline Peptone Water, for enrichment of Vibrio spp, Anaerobic Indicator tablet, Antibiotic Disc, Andrad~s Indicator, Antisera, Anti-Streptolysin O (ASLO) Rapid agglutination test, Etc.
Gram staining showed gram-positive nocardioform rods, and subculture on
tryptic soy agar with 7% sheep blood yielded polymorphous colonies that sunk into the agar and showed filamentous growth.
Line of ProteoMass MALDI-MS kits include: the Peptide and Protein kit for molecular weights between 757 Da and 66,430 Da; the Peptide kit for the typical mass range of
tryptic digestion fragments in reflectron mode (757 Da to 3,494 Da); and the Protein kit for typical protein range of 5,730 Da to 66,430 Da.
An accessories kit is also available for
tryptic digestion of proteins before reagent use and for cleanup of peptides afterward.
Liu and Bowers developed a solid-phase immunoaffinity trapping technique with mass spectrometric detection of
tryptic peptides to identify hCG in a urine sample fortified with 25 IU/L intact hCG (19).
Supply of chemical reagents to meet the needs of Laboratory Medicine, Biopathology (eg,
tryptic soy broth, pplo agar, anaerobic agar, etc.
Scientists at the University of Florida have identified specific biomarkers produced during the growth of the bacteria in
tryptic soy broth at 37 C which could be used to rapidly enumerate E.
Shot-gun" proteomics for complex mixtures of
tryptic peptides
