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Tryptic peptide sample reconstituted with water containing 0.1% formic acid was separated by a Nano Acquity UPLC system (Waters, USA).
The mCIM substituted 400 [micro]l of water for 2 ml of tryptic soy broth, adding just 1 [micro]l of organism instead of 10 [micro]l and extending the incubation period from 2 h to 4 h [+ or-] 15 min.
Tryptic peptide samples were reconstituted in 15 [micro]L of 3% acetonitrile with 0.1% trifluoroacetic acid.
In conclusion, we demonstrated that digested tryptic protein samples, which contain encrypted peptides, are able to exert an antiproliferative activity on neoplastic cells.
Phosphorylation sites on the detected tryptic peptides are shown in Figure S2.
Bacterial strains were sub-cultured in Tryptic Soy Broth (TSB, Merck, Germany) and further incubated for 48 h at 37[degrees]C.
E.tarda can be isolated on Edwardsiella Isolation Media (EIM), Brain Heart Infusion (BHI), Tryptic Soya Agar (TSA), Xylose Lysine Deoxycholate(XLD) and MacConkey.
Characterization of alpha-casozepine, a tryptic peptide from bovine alpha(s1)-casein with benzodiazepine-like activity.
Following alkylation, protein solutions were subjected to tryptic digestion, by the addition of 2.5 Og of sequencing grade porcine trypsin from Promega(R).
In previous works we demonstrated that these complexes have properties that partly overlap those displayed by native ones, being also characterized by an irreversible binding that confers on these complexes a particular resistance to tryptic digestion .
Isolation and identification: Samples were inoculated in Tryptic Soy Broth (TSB) and incubated at 37C for 24 hours.
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- Trypanosoma ignotum
- Trypanosoma lewisi
- Trypanosoma melophagium
- Trypanosoma neotomae
- Trypanosoma noctuae
- Trypanosoma rangeli
- Trypanosoma theileri
- trypanosome fever
- trypsin inhibitor
- tryptophan 2,3-dioxygenase
- tryptophan malabsorption syndrome
- tryptophan synthase
- tryptophanuria with dwarfism
- Tschemarke's treatment
- Tscherning ellipse
- tsetse fly
- TSH assay
- TSH test
- T-shaped fracture
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- Trypsin-Sensitive Counter Blocker
- Trypsinized Human Group O Erythrocytes
- Trypsinogen Like Immunoreactivity
- Tryptamines I Have Known and Loved
- Tryptic Phytone Glucose Agar
- Tryptic Soy Agar
- Tryptic Soy Broth Agar
- tryptic soy broth supplemented with yeast extract
- Tryptic Soy Broth Yeast Extract
- Trypticase Peptone Glucose Yeast
- Trypticase Soy
- Trypticase Soy Broth
- Trypticase Soy Broth with Yeast extract
- Trypticase-Blood-Sorbitol-CaCO3 Agar
- Trypticase-Yeast Extract
- Tryptone Bile X-Glucuronide
- Tryptone Glucose Extract Agar
- Tryptone Glucose Yeast Extract Agar
- Tryptone Glucose Yeast Extract Chloramphenicol
- Tryptone Soya Agar
- Tryptone Soya Yeast Extract
- Tryptone Soya Yeast Extract Agar
- Tryptone Yeast Glucose Agar
- Tryptone-Glucose-Yeast Extract