Albuquerque, In vitro activity of Rutaceae species against the trypomastigote
form of Trypanosoma cruz', Journal of Ethnopharmacology, 73(1- 2),335-340 (2000)
The bloodstream trypomastigote
form of the parasite was maintained in Swiss mice by serial passage of infected blood (11).
Taking into account the different motifs identified in the LYT1 UTRs and the roles played in other organisms, it can be hypothesized the following regulatory model: The Sxl element would have a positive regulation on the mLYTl transcript in the trypomastigote
stage whereas at the epimastigote stage this transcript would be down regulated by the G rich motif.
Leyenda: Figura 1: A: Imagen microscopica del microstrout en donde es posible visualizar un trypomastigote
metaciclico de Trypanosoma Cruzi senalado por la flecha.
During its life cycle, this parasite undergoes major changes in the kinetoplast morphology and kDNA topology as it passes from the epimastigote replicative stage in the insect vector to the metacyclic trypomastigote
form, which infects humans, in a process known as metacyclogenesis.
Infections were performed using trypomastigote
forms obtained at peak of parasitemia from previously infected Swiss mice (Waynforth and Flecknell, 1992).
Sanchez, "Identification of novel vaccine candidates for Chagas' disease by immunization with sequential fractions of a trypomastigote
cDNA expression library," Vaccine, vol.
Rosestolato, C.T., Dutra, J, M., De Souza, W., de Carvalho, T.M (2002) Participation of host cell actin filaments during interaction of trypomastigote
forms of Trypanosoma cruzi with host cells.
Although there is controversy among authors regarding the use of epimastigotes for these trials, it is important to note that previous studies demonstrated a correlation between epimastigote, trypomastigote
, and amastigote forms of T.
An enzyme immunoassay was reactive and an immunoblot of trypomastigote
excreted-secreted antigens was positive, confirming the diagnosis.
During week 29, routine monitoring was reinitiated and the patient tested positive by PCR, hemoculture, and serology (trypomastigote
excreted-secreted antigen [TESA] immunoblot).
Also, the LQB 123 compound presented greater selective action for the parasite highly infective trypomastigote
forms with selectivity index higher than 16 for bloodstream trypomastigotes
and higher than 21 for metacyclic trypomastigotes