The cell numbers and viability rates were determined after trypan blue
LDH assay estimated lower percentage cell viability than trypan blue
The cytotoxic effects of grape seed extract (GSE) on the growth of oral squamous cell carcinoma (KB cell line) were determined by MTT and trypan blue
assays as shown in Figures 1 and 2.
Cell viability, measured by trypan blue
exclusion assay, was 92.
Assessments of membrane integrity with vital dye, such as trypan blue
are nonspecific to determine the mode of cell death such as apoptotic cells unless morphological characteristics can be analyzed with the dyes (Todd et al.
Trypsin and trypan blue
were obtained from Himedia Labs, Mumbai, India.
Parameters assessed were cell morphology by phase-contrast microscopy, loss of adherence, and viability using a trypan blue
5] hemocytes (aliquots of cells from the same lobster), wells containing hemocytes plus noncytolethal (by trypan blue
exclusion) dilutions of putative NADPH oxidase stimuli, wells containing cells and 5 [micro]g/mL PMA and luminol or lucigen added to each well.
The cysts were then stained with Trypan blue
, a standard method for determining the viability of cysts since living cysts take up the dye at a much different rate than dead cysts.
Uptake of a vital dye, trypan blue
, by terminal follicles and total ovarian dry weight were used to assess the vitellogenic activity of the ovary, and SDS-polyacrylamide gel electrophoresis was employed to detect hemolymph Vg.
The viability of the cells was always >95% by the exclusion of trypan blue
Tenders are invited for Trypan Blue
For Intracameral Use