After sh-HULC and/or miR-130b mimic (inhibitor) transfection, B-E, the viability, migration, invasion, and apoptosis of GH3 cells, and F, the expression levels of PARP, cleaved-PARP, pro-caspase 3, cleaved-caspase 3, pro-caspase 9, and cleaved-caspase 9 in GH3 cells were assessed using
trypan blue staining assay, two-chamber transwell assay, Guava Nexin assay, and western blotting.
The
trypan blue and aceto-orcein staining revealed that the breakdown of nuclear membrane and starting of meiosis II reduced or stopped in a higher dose of DSAE-treated culture (Figure 4).
Strober, W.,
Trypan blue exclusion test of cell viability.
Then, viable fungal cells after exposure to caspofungin were determined by the
trypan blue assay.
After 24 h of incubation with fluorochrome-conjugated siRNA, cells were treated or not with
trypan blue before analysis.
Ramasamy, "A comparison of brilliant blue G,
trypan blue, and indocyanine green dyes to assist internal limiting membrane peeling during macular hole surgery," Retina, vol.
The total number of viable cells was determined at each time point by the
trypan blue exclusion test (Strober 2001).
Stem cells viability was tested by the standard
trypan blue method
Internal limiting membrane peeling with indocyanine green or
trypan blue in macular hole surgery: a randomized trial.
Today, agents such as indocyanine green,
trypan blue, autologous serum, triamcinolone acetonide, and brilliant blue are used to make the ILM more visible during MH surgery.
The cells viability was evaluated by means of dye exclusion test (0.04%
trypan blue solution).
Determination of cell growth: Cell density of hybridoma cells suspension was determined by
trypan blue exclusion method using a hemacytometer.
