The cell numbers and viability rates were determined after
trypan blue staining.
LDH assay estimated lower percentage cell viability than
trypan blue assay.
The cytotoxic effects of grape seed extract (GSE) on the growth of oral squamous cell carcinoma (KB cell line) were determined by MTT and
trypan blue assays as shown in Figures 1 and 2.
Cell viability, measured by
trypan blue exclusion assay, was 92.
Assessments of membrane integrity with vital dye, such as
trypan blue are nonspecific to determine the mode of cell death such as apoptotic cells unless morphological characteristics can be analyzed with the dyes (Todd et al.
Trypsin and
trypan blue were obtained from Himedia Labs, Mumbai, India.
Parameters assessed were cell morphology by phase-contrast microscopy, loss of adherence, and viability using a
trypan blue exclusion assay.
5] hemocytes (aliquots of cells from the same lobster), wells containing hemocytes plus noncytolethal (by
trypan blue exclusion) dilutions of putative NADPH oxidase stimuli, wells containing cells and 5 [micro]g/mL PMA and luminol or lucigen added to each well.
The cysts were then stained with
Trypan blue, a standard method for determining the viability of cysts since living cysts take up the dye at a much different rate than dead cysts.
Uptake of a vital dye,
trypan blue, by terminal follicles and total ovarian dry weight were used to assess the vitellogenic activity of the ovary, and SDS-polyacrylamide gel electrophoresis was employed to detect hemolymph Vg.
The viability of the cells was always >95% by the exclusion of
trypan blue dye.
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