This instrument also requires prior staining with
trypan blue.
Annotine at 100 [micro]g/ml did not affect viability of mDCs as assessed by
trypan blue staining and counting live cells (data not shown).
After 1, 2, 3 and 4 hours of heat exposure, the least loss of MDA-MB 231 viability was measured by
trypan blue meanwhile the highest was determined by crystal violet assay (Fig.
Splenocyte viability was consistently >95% as determined by
trypan blue exclusion.
cells cultured in the standard medium using
Trypan blue exclusion.
0 mm clear corneal incision was made and a continuous curvilinear capsulorrhexis was created, using
trypan blue if necessary, following which hydrodissection was performed.
Moreover, the percentage of dead sperm has been estimated by dyeing with
trypan blue (Ceballos-Vazquez et al.
As anti-D sera is added to Rh(D) RBCs, the antibodies are able to disrupt the RBC membrane which allows
trypan blue inside the cell providing visualization and quantification of RBC membrane destruction.
For counting the samples were mixed with
Trypan blue in equal volume.
We counted cells with a hemocytometer and assessed their viability by
trypan blue exclusion.
The viability and concentration of cells in suspension were measured by using the
Trypan blue dye exclusion method and the adhered cells were quantified by counting the stained nuclei with crystal violet.
Using successive pulses from two lasers, one with a wavelength of 1064 nanometres and the other radiating at 532 nanometres, the team rapidly heated a sample of fluid containing the dye
trypan blue.
