A salt or ester of tropic acid.
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Briefly, the extraction and derivatization procedures are as follows: Urine or aqueous solutions (1.5 mL) with internal standards tropate and 2-ketocaproate added are treated with hydroxylamine hydrochloride (30 min at 60 [degrees]C), acidified to pH 1 with HCI, saturated with NaCl, and extracted three times with 6 mL of ethyl acetate.
The relative recoveries were calculated on the basis of the monitored ion abundance expressed as a proportion of the internal standard (tropate, m/z 280).
The specimens for which creatinine was <2 mmol/L (concentration chosen arbitrarily) yield a small but statistically significant (P <0.001) decreased response for the internal standards 2-ketocaproate and 2-hydroxyvalerate vs that for tropate. For example, the abundance ratio of m/z 247 for 2-ketocaproate to m/z 280 for tropate is 0.41 [+ or -] 0.028 (mean [+ or -] SD; n = 26) when compared with more concentrated specimens (abundance ratio, 0.45 [+ or -] 0.038; n = 33).