The product was purified by column chromatography; eluting with hexane: ethyl acetate to give a white solid, triturating
with minimum amount of propan-2-ol to give the pure compound with 50% yield.
The mosquitoes were then tested for virus by triturating
them in BA-1 diluent as described for the Vero cell plaque assay, and injecting supernatants from the triturates into Vero cell cultures.
Cells are harvested from the broth overlay by adding fresh HS or HSA broth and triturating
to suspend the cells in a final volume of 7.
Perhaps polysaccharide hydrolyzing enzymes play a more crucial role in smaller shellfish that have less robust styles than mature adults, in which the mechanical triturating
of food by the grinding action of the style against the gastric shield plays a more important role.
Samples were prepared by triturating
100 mg of cross-linked and native biomaterials with 10% sodium dodecyl sulphate (1 ml) and supernatant was obtained after centrifugation at 10,000 rpm for 10 minutes.
The resulting product was isolated by adding methanol and triturating
, following by filtration and was dried at 80[degrees]C for 10 h under vacuum to leave 0.
The cuticular intima of the foregut in insects displays teeth, spines, piercing stylets and complex triturating
devices which apparently breakdown and filter food material (Snodgrass 1935; Parsons 1972; McGreevy et al.
Brash, rash, and violent in his plays on words and triturating
arguments, Novarina is relentless as well in his attempts to beat down the doors separating God's infinitude from the finitude of His creatures.
marinus stock culture was determined by triturating
cells through a 25-gauge needle and 5-mL syringe to break-up clusters prior to performing replicate hemocytometer counts.