tritiated thymidine

trit·i·at·ed thy·mi·dine

thymidine containing the hydrogen α-emitting radionuclide, tritium (3H or hydrogen-3); used as a marker to measure and localize by radioautography the synthesis of DNA, into which it is incorporated.

tritiated thymidine

(trĭt′ē-āt″ĭd thī′mĭ-dēn″, trĭsh′)
3H-Tdr; a radioactively labeled nucleoside used to measure T lymphocyte proliferation in vitro. Thymidine is essential for DNA synthesis; thus the amount of 3H-Tdr taken up is a general measure of the number of new lymphocytes produced.
References in periodicals archive ?
g/100mL) (n = 138) 14.07 2.08 10.9-22.8 Oxyhemoglobin (%) (n = 138) 51.23 18.27 23.3-99.9 Sample with low oxyhemoglobin -- -- -- level (%) ([dagger]) (n = 138) Oxygen content (mL/dL) (n = 138) 9.56 3.42 4.09-19.7 Methemoglobin (%) (n = 138) 0.57 0.37 0-1.65 Carboxyhemoglobin (%) (n = 138) 0.53 1.35 0-7.4 MTT (mitochondrial activity/optical 0.524 0.156 0.057- density; stimulatory index) (n = 122) 0.960 [sup.3]H, tritiated thymidine 21.5 27.01 0.60-144 (DNA synthesis activity; stimulatory index) (n = 100) IL-2, interleukin 2 586 952 36-4992 (concentration; pcg/mL) (n = 76) TNF-[beta], tumor necrosis factor 3486 3970 70-14,932 (concentration; pcg/mL) (n = 92) IL-10, interleukin 10 1079 1029 86-5789 (concentration; pcg/mL) (n = 92) Demographic % Hemoglobin (tot.
Cultures from old donors produced less T cell growth factor (TCGF) and incorporated less tritiated thymidine (3H-Tdr) than did similar cultures from young donors in the presence of either mitogen.
(19,20) In a prior study, (21) using tritiated thymidine labeling to identify S-phase cells, evidence of regional variation within breast carcinomas was detected in 61% of 50 tumors.
We were interested in examining the relationship between a woman's age and micronutrient deficiency status and how these factors contribute to an individual's overall immune function using two different measurements: their lymphocyte's antioxidant function (as measured by the ability of their lymphocytes to handle an oxidative challenge by treating the cells with hydrogen peroxide) and the overall ability of their lymphocytes to grow and proliferate (which we measured using a method called tritiated thymidine incorporation, which is a standard approach in immunoassays).
Using a tritiated thymidine assay, dose-dependent DNA synthesis inhibition was seen as early as 2 hours after treatment.
Catabolism of tritiated thymidine by aquatic microbial communities and incorporation of tritium into RNA and protein.
The cells used for the evaluation was human umbilical vein endothelial cells (HUVEC) and the proliferation rate of these cells on drug coated stent was compared against an uncoated stent made of the same base material as measured by the tritiated thymidine uptake of cells grown on these devices for period of 72h.
We obtained auricular LN cells and PBMC for each exposure group 48 hr after the final application of chemical, and the lympho-proliferative response was measured by tritiated thymidine incorporation at designated time points.
Virus production rates have also been estimated directly, by three independent methods: (a) tritiated thymidine incorporation into viral DNA (Steward et al., 1992), (b) dilution of fluorescently labeled viruses added to trace production and loss (Noble and Fuhrman, 2000), and (c) virus increase in samples filtered to reduce viral abundance (Wilhelm et al., 2002).
First, the determination of bacterial secondary production from incorporation rates of tritiated thymidine or leucine, requires itself the adoption of several conversion factors.
Hughes (Proc Natl Acad Sci) explain the organization and duplication of chromosomes using autoradiography with tritiated thymidine.
The incubation medium contains tritiated thymidine. As the cells proliferate, the tritiated thymidine is taken from the medium by the dividing cells and incorporated into their genetic material.