DAP I by 429%, DAP II by 355% and DAP IV by 178%, followed by cathepsin L (246%), cathepsin D (123%), tripeptidyl aminopeptidase (109%), arginyl aminopeptidase (78%), alanyl aminopeptidase (72%), leucyl aminopeptidase (49%), cathepsin H (37%) and proline endopeptidase (19%).
Tripeptidyl aminopeptidase: 50 mM Tris-acetate buffer pH 7.5, 2 mM DTT, 0.25 mM Ala-Ala-Phe-AMC.
On the other hand, head contained the highest activities of arginyl aminopeptidase (58 nmol/h/mg protein), leucyl aminopeptidase (49 nmol/h/mg protein), tripeptidyl aminopeptidase (51 nmol/h/mg protein) and proline endopeptidase (111 nmol/h/mg protein) than all the remaining body compartments.
Thus alanyl aminopeptidase, arginyl aminopepetidase, leucyl aminopepetidase, dipepetidyl aminopeptidase IV, tripeptidyl aminopeptidase and proline endopeptidase were increased by 72%, 78%, 49%, 178%, 109% and 19%, respectively.
Thus alanyl aminopeptidase was increased by 53%, arginyl aminopeptidase by 73%, leucyl aminopeptidase by 16%, dipeptidyl aminopeptidase IV by 17%, tripeptidyl aminopeptidase by 148% and proline endopeptidase by 111%.
Thus tripeptidyl aminopeptidase, proline endopeptidase and soluble proteins were increased by 58%, 61% and 24%, respectively while activities of arginyl aminopeptidase and leucyl aminopeptidase were decreased by 37% and 72%, respectively.
In dead flies these proteases manifested mixed response by showing further decreasing trend (such as arginyl and leucyl aminopeptidases and cathepsin L), increasing trend (such as soluble proteins) or adopted the rout of adjustment before death (such as tripeptidyl aminopeptidase, proline endopeptidase, dipeptidyl aminopeptidase I and 11, cathepsin B, H and D).