Spot Accession number number Protein name M17 K22E Keratin, type II cytoskeletal 2 epidermal M18 Q6NTA2 HNRNPL protein M19 Q32Q12 Nucleoside diphosphate kinase M20 ALDOA Fructose-bisphosphate aldolase A M21 PEBP1 Phosphatidylethanolamine-binding protein 1 M22 PGAM1 Phosphoglycerate mutase 1 N13 GMFB Glia maturation factor beta N14 HSPB1 Heat shock protein beta-1 N15 TP1S Triosephosphate isomerase
M23 PGAM1 Phosphoglycerate mutase 1 M24 ENOA Alpha-enolase Spot Theoretical Theoretical Number of number Gene name Mr pl peptide Score M17 KRT2 65,678.
32 (Fumarase) 6226 P00939 Triosephosphate isomerase
bieneusi genotypes in the fecal specimens and differentiated them by using PCR and sequence analysis of the small subunit rRNA gene (5), triosephosphate isomerase
gene (6), and ribosomal internal transcribed spacer (4), respectively.
As shown in the histogram of Figure 2, one protein from the SwissProt protein database matches the peptides of the mgf file with higher confidence than any other--in this case, triosephosphate isomerase
(TPI) has a protein score of 253, well above the probability cut-off (green shaded box) of 28 (P < 0.
McKenna and Farrell (2010) used DNA sequences from 9 nuclear genes (elongation factor-1[alpha] (EF-1[alpha]), alanyl-tRNA synthetase (AATS), CAD, 6-phosphogluconate dehydrogenase (PGD), sans fille (SNF), triosephosphate isomerase
(TPI), RNA Pol II, 28S, and 18S) to reconstruct the phylogeny of Holometabola with a focus on determining the phylogenetic placement of Strepsiptera.
A gene-rich cluster between the CD4 and triosephosphate isomerase
genes at human chromosome 12p13.
ta(2,4)-dienoyl-CoA isomerase, mitochondrial, platelet-activating factor acetylhydrolase IB subunit beta, endoplasmic reticulum protein ERP29 (ERP29), protea-some subunit beta type-6, fructose-bisphosphate aldolase A, triosephosphate isomerase
(TIM), and D-3-phosphoglycerate dehydrogenase (3-PGDH), while down-regulated proteins are TAR DNA-binding protein 43 (TDP-43), splicing factor, arginine/serinerich 1 (SF2/ASF), cDNA FLJ55253 highly similar to actin cytoplasmic 1, eukaryotic translation initiation factor 3 subunit I (eIF3i), and actin aortic smooth muscle.
In case of spots which displayed reduced expression levels, structural proteins like Septin-2, cofilin-1 and macrophage capping protein; proteins involved in cellular processes like Annexin 1, Annexin 4, Annexin 5 and proteins involved in metabolism like phosphoglycerate mutase, triosephosphate isomerase
, peptidyl-prolyl cis-trans isomerase were identified (Figure 5; Table 2).
To address the source of infection in humans and public health importance of Giardia duodenalis parasites from animals, nucleotide sequences of the triosephosphate isomerase
(TPI) gene were generated for 37 human isolates 15 dog isolates, 8 muskrat isolates, 7 isolates each from cattle and beavers, and 1 isolate each from a rat and a rabbit.
36) showed that biofilms of Staphylococcus aureus were upregulated for genes encoding enzymes involved in glycolysis or fermentation (phosphoglycerate mutase, triosephosphate isomerase
, and alcohol dehydrogenase) and surmised that the up-regulation of these genes could be due to oxygen limitation in the developed biofilm, favoring fermentation.
b) ADHE = Aldehyde-alcohol dehydrogenase; AT = Aconitase; FBA = Fructose-bisphosphate aldolase; FR = Fumarate reductase; GAPDH = Glyceraldehydes 3-phosphate dehydrogenase; GPI = Glucose-6-phosphate isomerase; HK = Hexokinase; ICD = Isocitrate dehydrogenase; IPGM = 2,3- bisphosphoglycerate-independent phosphoglycerate mutase; LD = D- lactate dehydrogenase; MD = Malate dehydrogenase; ME = Malic enzyme; PEPCK = Phosphoenolpyruvate carboxykinase; PFK = Phosphofructokinase; PFL = Pyruvate formate lyase; PFLA = Pyruvate formate lyase activating enzyme; PGK = 3-phosphoglycerate kinase; PK = Pyruvate kinase; PPS = Phosphoenolpyruvate synthase; SCSB = Succinyl-CoA synthetase, beta subunit; TIM = Putative triosephosphate isomerase
Thus, the higher expression of aldolase B, glyceraldehyde-3-P dehydrogenase, 6-phosphofructo-2-kinase, and triosephosphate isomerase
are actually consistent with the hepatic focus on gluconeogenesis.