The protein marker consisted of triosephosphate isomerase
from rabbit muscle (26.6 kDa), myoglobin from horse heart (17.0 kDa), [alpha]-lactalbumin from bovine milk (14.2 kDa), aprotinin from bovine lung (6.5 kDa), insulin chain B, oxidized, bovine (3.496 kDa), and bradykinin (1.06 kDa).
Castillo-Villanueva et al., "Giardial triosephosphate isomerase
as possible target of the cytotoxic effect of omeprazole in Giardia lamblia," Antimicrobial Agents and Chemotherapy, vol.
aureus as described previously, including carbamate kinase (arcC), shikimate dehydrogenase (aroE), glycerol kinase (glp), Guanylate kinase (gmk), phosphate acetyltransferase (pta), triosephosphate isomerase
(tpi), and acetyl coenzyme A acetyltransferase (yqi).
The standard TIM coupled assay  contained glyceraldehyde-3-phosphate (0.4 mM), NADH (0.1 mM), [alpha]-glycerophosphate dehydrogenase (0.5 units/ mL), and triosephosphate isomerase
(rabbit muscle TIM) (0.01 units/mL) in 0.1 M triethanolamine hydrochloride buffer, pH 8.0 .
Crystal structures of P falciparum glycolytic enzymes aldolase (PfALDO) , triosephosphate isomerase
(PfTPI) , glyceraldehyde 3-phosphate dehydrogenase (PfGAPDH) , lactate dehydrogenase (PfLDH) , glucose 6-phosphate isomerase (PfGPI) (Gileadi et al.
Knowles, "Stabilization of a reaction intermediate as a catalytic device: definition of the functional role of the flexible loop in triosephosphate isomerase
," Biochemistry, vol.
farinae allergens, Der f 25 is a triosephosphate isomerase
(TPI) with a molecular weight of 34kDa, showing 75.6% by immunoblotting and 60% by skin prick positive reaction to dust mite allergic patients, respectively.
To our knowledge, only a limited number of enzymes with such catalytic carboxyl group(s) are known so far in other groups of enzymes than hydrolases and transferases, such as triosephosphate isomerase
(catalytic Glu), (201) glucosamine 6-phosphate isomerase (or deaminase) (catalytic Asp), (202) phosphofructokinase (catalytic Asp) 203) etc., where the catalytic Glu/Asp residues are also thought to function as general acid/bases.
Amongst those identified they made an in vitro function study by silencing the fatty acid-binding protein, heart-type (FABP3), and triosephosphate isomerase
The isolates were identified by the rapid ID32A system (bioMerieux, Inc., Durham, North Carolina, United States of America) and polymerase chain reaction amplification of the triosephosphate isomerase
bieneusi genotypes in the fecal specimens and differentiated them by using PCR and sequence analysis of the small subunit rRNA gene (5), triosephosphate isomerase
gene (6), and ribosomal internal transcribed spacer (4), respectively.
McKenna and Farrell (2010) used DNA sequences from 9 nuclear genes (elongation factor-1[alpha] (EF-1[alpha]), alanyl-tRNA synthetase (AATS), CAD, 6-phosphogluconate dehydrogenase (PGD), sans fille (SNF), triosephosphate isomerase
(TPI), RNA Pol II, 28S, and 18S) to reconstruct the phylogeny of Holometabola with a focus on determining the phylogenetic placement of Strepsiptera.