For identification of collagen fibers, Masson's trichrome stain
was used in separate sections.
The sarcoplasmic rods were seen as small, clustered dark eosinophilic sarcoplasmic inclusions on hematoxylin and eosin stain and were red on Gomori trichrome stain
. No myonecrosis or inflammatory cell infiltrate was found (Figure 1).
The Masson's Trichrome stain
showed the collagen of the ECM is conserved in umbilical arteries after decellularization, with a more attenuated stain appreciated in Triton X-100 (Fig.
The islets were separated from the acinar group by a thin capsule made up of reticular fibres, clearly distinguished by Mallory trichrome stain
Results of stool microscopy performed using Ryan's modified trichrome stain
were negative for microsporidia.
A rapid one-step trichrome stain
. American Journal of Clinical Pathology 20:661-664.
To elucidate the fibrosis of lung parenchyma, Masson's trichrome stain
was performed according to the manufacturer's instruction.
Masson's trichrome stain
was used for collagen and proteoglycans.
They can be visualized on light microscopy by the Gomori trichrome stain
, appearing as dark blue structures localized in the sarcoplasm, predominantly in regions with disrupted sarcomere structure.
Caption: Figure 1: (a) Liver autopsy of case 1 showing disseminated lipid accumulation and severe steatosis with lipid-filled histiocytes (arrows) (Masson trichrome stain
(d) Trichrome stain
with focal mild interstitial fibrosis and tubular atrophy (e) Electron microscopy.
(c) Frozen section, Gomori trichrome stain
, revealed occasional rimmed vacuoles (arrow).