Bone marrow aspiration and
trephine biopsy findings were consistent with a diagnosis of severe aplastic anemia (marrow cellularity=20% for age; Figure 2a-g).
We reported a case of refractory classical Hodgkin lymphoma in which Hodgkin Reed-Sternberg (HRS) cells were only demonstrated in bone marrow aspirations and
trephine biopsy sections only when the disease disseminated.
Eighty-five of the benign cases (66%) and 76 malignant cases (97%) had aggregates present in the
trephine biopsy sections, which permitted accurate evaluation for the presence of paratrabecular localization.
Optimal processing of bone marrow
trephine biopsy: The Hammersmith Protocol.
Objective: To determine the usefulness of bone marrow aspiration and
trephine biopsy in evaluation of the bone marrow in routine haematological practice.
Bone marrow aspirate and
trephine biopsy showed no neoplastic infiltration.
Bone marrow aspiration (BMA) and
trephine biopsy were obtained from all patients at diagnosis and also from controls.
Bone marrow (BM)
trephine biopsy and immunophenotype showed extensive infiltration from B-ALL expressing the surface markers CD10, CD19, CD22, CD79a, CD34, CD123, CD38, and Tdt, with an aberrant coexpression of the myeloid markers CD13, CD33.
Both patients had hematological findings characteristic of the accelerated phase of Chediak-Higashi syndrome-peripheral blood pancytopenia and marked hemophagocytosis (Figure 2) on bone marrow aspirate, along with lymphocyte infiltration on
trephine biopsy section.
Relevant clinical details, investigations, associated morphological features on bone marrow aspirate and
trephine biopsy (wherever it was performed), and residential address along with history of visit to endemic regions were recorded for every case.
With this information it is logical to directly examine the bone marrow by means of aspiration coupled with
trephine biopsy (Fig.
Their approach is based on the integrated assessment of peripheral blood and bone marrow aspirate films,
trephine biopsy sections, and supplementary investigations, and includes discussion of the clinical context of bone marrow diagnosis and cytological and histological features.
