In the present study, we determined the changes in the total carbohydrate, glycogen, trehalose, glycerol and protein contents, as well as esterase and trehalase
activities of H.
Joao Martins Pizauro Junior (Unesp-Jaboticabal) for his technical support on the purification of the trehalase
used in this work.
activity was determined by the 3,5-dinitrosalicylic acid colorimetric method (DNS method) (Miller 1959; Wang 2004) with absorbance measured at 540 nm.
1959); amylase as given in Wootton and Freeman (1982) and trehalase
by the procedure of Dahlqvist (1966).
also contributes [alpha]-glycosidase activity (Kreikemeier et al.
This chapter would have been improved with a brief mention of other malabsorption syndromes such as intestinal lymphangiectasia, transporter defects such as oasthouse syndrome and enzyme deficiencies such a trehalase
and sucrase isomaltase deficiency The section on anorexia nervosa seems to be predominantly a psychological overview.
1999) as well as b-glucosidase and trehalase
inhibitory activities (Asano et al.
Alpha-amylase is secreted by the pancreas, while isomaltase, maltase-glucoamylase, trehalase
and lactase are secreted by the intestinal mucosa (Harmon, 1993).
The glucose thus formed in the intestine probably is absorbed to be utilized as an energy source because patients deficient in intestinal trehalase
have been reported to show diarrhea after ingestion of trehalose-containing mushrooms (1).
Researchers have developed processes for preparing high-purity trehalase
concentrates, including a fermentation strategy that maximizes basal nonrepressed trehalase
Honek is interested in the design, synthesis and enzymology of novel enzyme inhibitors, particularly with respect to the biological chemistry of methionine and glycohydrolytic enzymes such as trehalase
melanogaster are the four negative correlations with trehalase