Trehalase activity in T dendrolimi adults grown in vitro and in vivo were basically the same over 10 generations.
Trehalase is a specific enzyme in glucose metabolism of insects.
Trehalase activity in Trichogramma dendrolimi reared in vitro and in vivo for 10 generations.
Brush border membrane sucrase-isomaltase, maltase-glucoamylase and trehalase
Once scientists fully investigate the potential for trehalase
induction, they will perform pilot fermentations for making sufficient quantities for an initial commercial trial.
Mammalian trehalase is linked to membrane by a glycosylphosphatidylinositol anchor, and phosphatidylinositol-specific phospholipase C is required for the trehalase to be completely liberated from the membrane (5).
Trehalase activity in urine has been estimated by determination of formed glucose after the removal of endogenous glucose from urinary samples by complicated gel filtration (3,7).
Demonstration of functional expression of human trehalase in Escherichia coli (9) facilitates the isolation of monoclonal antibody for human trehalase that can be used to develop an ELISA method.
Monoclonal antibodies against human trehalase were raised using recombinant trehalase and synthesized trehalase peptide.
Since the energy supply of vertebrates is provided by the metabolic pathway of trehalose, and because this disacharide is critically important in insect metabolism, trehalase may be developed as a specific target of new biopesticides.
Here, we report the cloning and characterization of the membrane-bound trehalase cDNA BdTre2 in B.
For use as outgroups, a total of 36 insect trehalase genes were obtained from GenBank, including 16 Tre2, 14 Tre1 and another 6 trehalase genes from microbes and mammals (Table 2).