cpl] 26 transposase
ISEcp1R TTTCCGCAGCACCGTTTGC [ISE.
Transposagen tested over 2 million variations of the piggyBacTM transposase
enzyme and discovered versions of the enzyme that are more than 10 times more active than the version found in nature.
After excluding single-nucleotide polymorphisms (SNPs) in genes annotated as PE/PPE, integrase, transposase
, or phage and SNPs that showed an ambiguous base call, we kept 12,920 high-confidence variable positions for downstream analysis.
This technology uses a transposase
enzyme complexed with a transposon, which, in this case, contains Illumina adapters (product data sheet).
2010) together with transposase
mRNA (36 ng/[mu]L), and the injected embryos were raised to adulthood.
The PiggyBac process involves shipping cargos of genetic material - called transposons - around the genome using an engine known as a transposase
They possess an enzyme called a transposase
which enables them to cut-and-paste themselves into new sites in the genome.
We are constructing both a piggyBac vector and transposase
source for transformation into the Zebrafish, Danio rerio, by the microinjection method.
Elements of Class II transpose via DNA intermediate catalyzed by transposase
encoded by autonomous element.
Some naturally occurring control systems are based on this mechanism, such as the control the expression of the transposase
of IS10 in bacteria (Ma & Simons 1990) or the N-myc gene in human cells (Krystal et al.
The transposon, which in this study consists of DNA engineered from a fish gene, encodes an enzyme called transposase
While piggyBac relies on what's called a transposase
enzyme to help it move, tagalong lacks this enzyme and relies on something else to help it travel.