Providing LPXTG tagged antibodies (A penta-peptide as a substrate for transpeptidation
reaction) as specific linkage sites for the oligo-glycine-containing payloads, which are mediated by Staphylococcus aureus Sortase A enzyme (186),
It interferes with the bacterial cell wall synthesis and inhibits the enzyme (DD-transpeptidases) which is responsible for the transpeptidation
reaction; a vital step to maintain the integrity of cell wall (Chowdhury and Lieberman, 1999).
Through transglycosylation, the pentapeptide is incorporated into peptidoglycan and via transpeptidation
crossbridges form to strengthen the peptidoglycan layer.8
In staphylococci, vancomycin, for example, inhibits the second stage of CW synthesis, blocking the transglycosylation step, which subsequently affects transpeptidation
Mechanism of heavy metal ion activation of phytochelatin (PC) synthase blocked thiols are sufficient for PC synthase-catalyzed transpeptidation
of glutathione and related thiol peptides.
(7) Piperacillin, like other [beta]-lactam antibiotics, exerts bactericidal activity by inhibiting the final transpeptidation
step of peptidoglycan synthesis in bacterial cell walls, thus inhibiting cell wall biosynthesis of susceptible bacteria.
Their mechanisms have been proved via cell membranes perturbations coupled with the action of a-lactams on the transpeptidation
of the cell wall could lead to an enhanced antimicrobial effect of the combinations (15).