transfection

(redirected from transfections)
Also found in: Dictionary, Encyclopedia.
Related to transfections: immunohistochemistry

trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.

transfection

[-fek′shən]
Etymology: L, trans + inficere, to taint
the process by which a bacterial cell is infected with purified DNA or RNA isolated from a virus after a specific pretreatment. Acute transfection is short-term infection. transfect, v.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection (transfek´shən),

n the process by which a bacterial cell is infected with purified deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) isolated from a virus or a viral vector after a specific pretreatment.

transfection

an introduction of free DNA into a cell.
References in periodicals archive ?
Taking into consideration the nucleic acid types, size and function of messenger RNA, Oz Biosciences has developed new specific reagents allowing mRNA transfection with high efficiency.
A 5 | g transfection of a GFP-expressing plasmid served as a negative control.
In this study, we tried to optimize transfection of MSCs with GFP containing phGFP vector and then examined the fate of MSCs both in vitro and in vivo.
In a trial using CMV-LUC (4 [micro]L of 500 ng DNA/pL; n = 5), approximately 80-90% of the total brain luciferase activity was found in the telencephalon and optic tectum, whereas the hypothalamus and cerebellum plus hindbrain had very low levels of transfection (data not shown).
the pioneer in scalable, high performance transient transfection systems, is hosting an educational webinar entitled Transient Transfection of Biologically Relevant Cells: CEVEC CAP-T[TM] Technology for High Yield Expression of Proteins in Human Derived Cells on Wednesday, December 12, 2012, from 11:00 AM - 12:00 PM EST.
The presence of blue-stained cells after 24 hours indicated positive transfection.
who screened more than 2,300 candidate polymers to identify novel biodegradable nanoparticles that facilitate exceptional transfection efficiency.
Because the new TransCytos transfection technology is gentle, highly effective, and does not physically damage cells, it is potentially capable of transfecting all cell types," says Dr.
Traditional lipid-based siRNA transfection reagents exhibit a number of drawbacks, including high cell death rates and a limited ability to deliver siRNA into a variety of cell types, such as primary, neuronal, differentiated, and nondividing cells," said Tim Fleming, Director of Global Commercial Marketing at Sigma-Aldrich.
After transfection, the cells were treated with increasing doses (2.
It is now possible to easily scale up from single step tedious and labor intensive processes into hundreds or thousands of controlled transfections.