transfection


Also found in: Dictionary, Encyclopedia, Wikipedia.

trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]
References in periodicals archive ?
For trypan blue staining, after relevant transfection, GH3 cells were seeded into a 6-well plate (Thermo Fisher Scientific, USA) with 1 x [10.sup.5] cells per well and cultured at 37[degrees]C for 24 h.
Electroporation instruments allow for efficient transfection by inducing transient pores to form in the cell membrane in response to a carefully controlled electrical pulse.
After 12 hours, the miR-375 mimics, miR-375 inhibitor and miRNA-ShNC were transfected with FuGENE HD Transfection Reagent.
The day before transfection, the 5x[10.sup.3] cells were counted and seeded into 6-well plates.
Digital images of cells after transfection were obtained using the inverted Axiovert 200 fluorescent microscope, using the Axiovision 3.0 image analysis software.
Additionally, we overexpressed miR-34a in different colon cells by transfection of miR-34a mimic or Mimic NC.
Nowadays, Mirus Bio is the leading manufacturer of transfection reagents for chemical and/or electroporation delivery of DNA, RNA, siRNA, miRNA, and other nucleic acids.
We used the non-modified polyethylenimine (PEI) reagent which is a cheap and effective transfection reagent to optimize transfection protocols for HepG2 cell-lines, and transiently transfected a number of cell-lines up to 70-80%.