transfection

(redirected from transfect)
Also found in: Dictionary, Encyclopedia, Wikipedia.

trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]
Farlex Partner Medical Dictionary © Farlex 2012

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]
Medical Dictionary for the Dental Professions © Farlex 2012
References in periodicals archive ?
E14 murine ES cells were stably transfected with TET2-pCDNA3 plasmid vector (TET2 stable transfects) or empty pCDNA3 plasmid as a control (EV stable transfects) in triplicate.
For laboratories that need to universally transfect or transform all cell types of organisms, instruments that offer both square and exponential decay waveforms such as the BTX Gemini Twin Wave Electroporator offer both square and exponential decay modes for universal electroporation of any sample.
At four days after induction with DOX, transfected HTF cells were collected and lysed in buffer containing 150 mM NaCl, 50 mM Tris-HCl (pH 7.4), 2 mM EDTA, 1% NP-40, and protease inhibitors (Boehringer Mannheim, Germany).
As shown in Figure 1(a), healthy cells with the same membrane capacitance and resting potential (AP) were selected to determine the intrinsic electrophysiological properties of transfected neurons (Figures 1(b) and 1(c)).
The study recruited five patients with AIDS-related lymphoma, four of whose blood stem cells were harvested, transfected with the triple vector RNA therapeutic and then reinfused back into the patients.
Quantitative analysis revealed that FoxO1-AAA transfected cells exhibited ~8-fold ([+ or -]0.53; p < 0.01) increase of FoxO1 mRNA level, as compared to cells transfected with vector control (Figure 5(a)).
The plasmids (10 [micro]g) were transfected into Platinum-E packaging cells using X-tremeGENE 9 DNA transfection reagent (Roche).
Caption: FIGURE 2: Top: western analysis of PCNA protein levels in total cell extracts of the CL1-0 cells stably transfected with wild type PCNA (EGFP-wt.
Safranin was used to assess [DELTA][[psi].sub.m] in digitonin- permeabilized transfected cardiomyocytes as previously described by Oliveira et al.
To detect whether the expression of PPAR[gamma] could enhance its transcription activity, H9c2 cells were transfected with PPRE3luciferase reporter together with PPAR[gamma] plasmids.
In addition, MC3T3-E1 cells were transfected with SMAD5-expressing lentiviruses or control lentiviruses (Hanheng, Shanghai, China) at a multiplicity of infection (MOI) of 10 in 5 mg/mL Polybrene (Sigma-Aldrich) for 12 h followed by treatment with 10 [micro]g/mL puromycin (Santa Cruz Biotechnology, CA, USA) for 21 days according to the manufacturer's protocol.
458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals