transfection

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trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.

transfection

[-fek′shən]
Etymology: L, trans + inficere, to taint
the process by which a bacterial cell is infected with purified DNA or RNA isolated from a virus after a specific pretreatment. Acute transfection is short-term infection. transfect, v.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection (transfek´shən),

n the process by which a bacterial cell is infected with purified deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) isolated from a virus or a viral vector after a specific pretreatment.

transfection

an introduction of free DNA into a cell.
References in periodicals archive ?
Transfect (transfection): refirring to the introduction and incorporation of outside DNA (eg, from a virus) into a cell.
We constructed and used TB-4 recombinant AAV to transfect human NP cells directly and have clearly demonstrated that our recombinant AAV transfection technique was able to highly and stably express TB-4 in human NP cells, which may provide a new pathway for innovation in the treatment of IVD degenerative diseases in the field of practical regenerative medicine.
has launched its VLX Large Scale Transfection System with the capability to transfect up to 2E11 cells in less than 30 minutes with high efficiency and high viability, dramatically extending the gram-scale protein bioproduction capabilities of the current MaxCyte instrument product line.
Many of these cell lines that we'd like to use in a cell culture-based vaccine are very hard to transfect with plasmids," says Karen Lacourciere, an influenza program officer at the National Institute of Allergy and Infectious Diseases.
Stem cells and primary cells have traditionally proven to be very difficult to transfect.
The highlighted event will be the introduction of the MaxCyte[R] VLX[TM] Large Scale Transfection System which has the capability to transfect up to 2E11 cells in less than 30 minutes with high efficiency and high viability, dramatically extending the gram-scale protein bioproduction capabilities of the current MaxCyte instrument product line.
Three comer-cialized transfection reagents, Lipofectamine 2000 (lipoplex) (Invitrogen, CA, USA), jetPEI (polyplex) (Polyplus, Paris, France) and LyoVec (lipoplex) (Invivogen, CA, USA) were used to transfect the cell lines based on the manufacturer's recommendation.
The X-tremeGENE 9 reagent offers efficient performance across a wide variety of commonly used cell lines while Roche's X-tremeGENE HP reagent is especially well suited for cell lines that are difficult to transfect or not easily transfected with other reagents.
In addition to demonstrating the simple operation of the MaxCyte STX to transfect any cell with any molecule at any scale quickly and reproducibly, Dr.
The MaxCyte STX represents a fast, simple, and scalable way to transfect cell lines, primary cells, and stem cells with high efficiency and performance for cell-based assays for screening ion channels, GPCRs, and other targets," says Dr.
This is particularly valuable for stem cells and primary cells that are the most medically relevant and the most difficult to transfect.
The ability of the MaxCyte STX to transfect cells with ion channels and other hard-to-transfect targets is proving to be a valuable tool for pharmaceutical companies to accelerate the screening of compound libraries for drug candidate selection.