The Panther Fusion system adds the capacity to run polymerase chain reaction (PCR) assays to the transcription-mediated amplification
(TMA) assays that are performed on the base Panther system.
from the American Red Cross in Gaithersburg, Md., and colleagues implemented investigational screening of donated blood for ZIKV RNA with transcription-mediated amplification
Different isothermal molecule amplification assays have been developed as a rapid, simple, and cost-effective alternative to PCR-based molecule assay since 1990s, which include loop-mediated isothermal amplification assay (LAMP) , helicase-dependent amplification assay , rolling circle amplification assay , nucleic acid sequence-based amplification assay , transcription-mediated amplification
assay , signal-mediated amplification assay , self-sustained sequence replication assay , ramification amplification assay , and RPA assay .
(2-5) In a prospective study of 933 evaluable women, the Aptima test detected trichomonas RNA by transcription-mediated amplification
with a clinical sensitivity of 95.3% to 100% and specificity of 95.2% to 100% in a variety of specimens, including vaginal, urine, endocervical, and ThinPrep Pap(tm) samples.
A transcription-mediated amplification
(TMA) assay has been developed (9,10) which uses analyte-specific reagents (ASRs) for M.
A prototype CHIKV transcription-mediated amplification
(TMA) assay was used to screen blood donors from Puerto Rico during the peak of the 2014 Caribbean epidemic (Table).
NASBA and transcription-mediated amplification
(TMA), often referred to collectively as transcription-based amplification methods, are both isothermal RNA amplification methods modeled after retroviral replication (13, 14).
Screening of male patients for Trichomonas vaginalis with transcription-mediated amplification
in a community with a high prevalence of sexually transmitted infection.
The intensive and time-consuming reverse-transcriptase polymerase chain reaction PCA3 urine test has been translated successfully into the fast and easy transcription-mediated amplification
(TMA)-based PCA3 test.
APTIMA[R] Trichomonas vaginalis, a transcription-mediated amplification
assay for detection of Trichomonas vaginalis in urogenital specimens.
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification
; VRE = Vancomycin Resistant Enterococcus.
The residual urine samples from routine pregnancy testing were evaluated for chlamydia and gonorrhea with GenProbe's transcription-mediated amplification
testing, while testing for T.