transconjugant

transconjugant

(of bacteria) a recipient that inherits DNA from the DONOR during CONJUGATION.
References in periodicals archive ?
coli transconjugant back to a rifampin-resistant mutant of Salmonella Anatum strain R13.0957 (Appendix, https://wwwnc.cdc.gov/EID/ article/25/1/18-1103-App1.pdf).
The transconjugant presented elevated MIC for colistin in comparison with E.
Successful conjugation would transfer ESBL determining plasmid from donor (Cefotaxime Resistant-[Ctx.sup.R]) to recipient (Streptomycin Resistant-[Str.sup.R]) and the resultant product (Transconjugant) would hence be phenotypically [Str.sup.R] [Ctx.sup.R].
sullae strain towards the Pseudomonas aeruginosa strain on solid culture medium and to study the characters acquired by the transconjugants. Results The use of the chloramphenicol antibiotic and the calcofluor white as selection agents have made it possible to select transconjugant strains capable to produce the exopolysaccharides.
Transconjugant was selected on combined plates containing meropenem (1 mg/L) to inhibit the growth of recipient strain and rifampicin (256 mg/L) or sodium azide (100 mg/L) to suppress donor strains.
To corroborate the self-conjugation ability of the pEAF plasmids, the purified plasmid from a transconjugant colony derived from the first conjugation event was transformed by electroporation into DH5[alpha] using kanamycin as selection marker.
Transconjugant strains showed the same elevation of carbapenem MICs as exhibited by donor strains.
A single colony of each transconjugant was left to grow for 16 h on MacConkey agar and was placed in 200 [micro]L sterile water in a 1.5 mL microtube.
Nolan, "Characterization of a series of transconjugant mutants of an avian pathogenic Escherichia coli isolate for resistance to serum complement," Avian Diseases, vol.
Parallel to the above experiments, in vitro the vector plasmid pBR322 DNA cloning was used to select and to get quicker auxotrophic E.coli K-12 hybrid transconjugant. All the above experiments were routine wise to isolate one GE 5405 E.coli K-12 to study the cause of expressions of donor MRHU (+) plasmids into E.coli K-12 auxotrophic phenogenotypes and thereby the changing surface antigenic properties of E.coli K-12, expressing BNT= fimbriae =pili on the outer most cell wall of E.coli K-12 at auxotrophic pheno-genotypes, [6,7].
Soybean transformations were conducted with the resultant transconjugant as previously described (Zhang et al., 1999; Clemente et al., 2000).
Plasmid pB 15 was moved into [[Ara.sup.-].sub.0] and [[Ara.sup.+].sub.0] by mixing each strain with a donor and selecting a transconjugant after overnight matings.
Full browser ?