Intergenically spliced chimeric RNAs have been shown to occur via cis-splicing of adjacent genes (cis-SAGe) as well as long-range intrachromosomal and interchromosomal trans-splicing events [3, 5, 6].
Further characterization of SLC45A3ELK4 chimeras showed that the transcript was created through cis-SAGe rather than trans-splicing , and most notably the exon 1/exon 2 form of the chimera functions as an androgen-responsive chimeric long noncoding RNA  (Figure 1(a)).
As chimeric trans-splicing requires both parental transcripts to be present, it is likely that these events may be dependent upon the spatial proximity of the parental genes.
Lin, "Putative alternative trans-splicing
of leukocyte adhesion-GPCR pre-mRNAs generates functional chimeric receptors," FEBS Letters, vol.
Among the topics are yeast-based chemical genomic approaches, activity-based protein profiling of cys proteases, using analogue sensitive technologies to target kinases of interest, using split inteins to prepare semi-synthetic proteins and to study the mechanisms of protein trans-splicing
, and introducing chemical reporter groups by bio-orthogonal ligation reactions for imaging cell-surface glycans.
group I intron and tRNA-hyperediting in the mitochondrial genome of the lycophyte Isoetes engelmannii.
A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing
ribozymes of the invention.