The main objective of the presented research was to analyze and assess toxinogenic capabilities of fungi isolated from moldy surfaces in residential rooms in a big urban agglomeration located in moderate climatic zone far away from flooded areas.
Toxinogenic capabilities of molds isolated in our study were analyzed according to the literature data [39-42].
Air samples and scrapings from moldy surfaces collected in flats where isolated was any of the 2 potentially toxinogenic (Aspergillus versicolor or Penicillium chrysogenum) species of fungi, were analyzed for the presence of mycotoxins (sterigmatocystin and roquefortine C) using the HPLC method.
To check the toxinogenic activity of the collected Aspergillus and Penicillium genera, the authors cultured them on microbiological media and on the building materials inoculated with the mycelium in the laboratory.
Escherichia coli strains are pathogenic and toxinogenic
. In particular one can recognize an enterohaemorrhagic strain causing very dangerous outbreaks (O157: H7: without pili "Ohne Haar") or RS218, causing meningitis.
IMB has long-standing experience in algal culture, and has recently developed technology for rapid bulk production of toxinogenic
Molecular epidemiology survey of toxinogenic
C.Perfringens strain types by multiplex PCR.' Scand J.Infect.Dis., 32: 637-641.
Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB (toxinB) genes for toxinogenic
culture of Clostridium difficile.
Polymerase chain reaction assay for diagnosis of potentially toxinogenic
Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay.
The 3 isolates were found to be toxinogenic
by PCR; 2 were PCR ribotype 017, and 1 was PCR ribotype 001 (Table 1).
On the other hand, deletions in tcdC are frequently present in toxinogenic
Subsequently, the Istituto Superiore di Sanita (ISS) in Rome confirmed isolation of toxinogenic