Awada and Daneault  prepared chemically modified PVA containing tosyl
, azide, and amin functional groups that were attached to the carbon backbone.
In the second step the tosyl
chloride was reacted with substituted benzimidazoles in basic conditions to afford the title N-tosyl benzimidazoles (4a-e).
HEPES, sodium chloride, ammonium formate, urea, tris base, iodoacetamide, and tosyl
lysyl chloromethyl ketone were purchased from Sigma Aldrich.
Ryousuke Hayashi, "Solution copolycondensation of isophthalic acid, terephthalic acid, 4,4/dihydroxydiphenylsulfone, and bisphenols with a tosyl
chloride/dimethylformamide/pyridine condensing agent," Journal of Applied Polymer Science, vol.
Pellacani, "Role of the tosyl
group in the coordination ability of N-protected amino acids.
The viruses were cultured for 2 days in Madin-Darby Canine Kidney (MDCK) cells with tosyl
sulfonyl phenylalanyl chloromethyl ketone(TPCK-) treated trypsin (2 [micro]g/ml) (Sigma, St.
N-a-Benzoyl-l-arginine ethyl ester (BAEE) and trypsin (TPCK (tosyl
phenylalanyl chloromethyl ketone) treated trypsin) were purchased from Sigma-Aldrich Co., USA: chitosan (CTS), iron(II) sulfate heptahydrate (Fe[O.sub.4] x 7[H.sub.2]O), 25% ammonia solution (N[H.sub.3] x [H.sub.2]O), iron(III) chloride hexahydrate (Fe[Cl.sub.3] x 6[H.sub.2]O), ammonium bicarbonate (N[H.sub.4]HC[O.sub.3]), acetic acid, glutaraldehyde, liquid paraffin, Tween-80, magnesium stearate, ethyl ether, acetone, ethanol, bovine serum albumin (BSA), and pure water (with an electrical resistivity of 18.2 M[ohm] cm).
With this background, the substitution of the C3-OH of (2R,3S) 5,7,3',4'-tetramethoxyflavan-3-ol (catechin) 8 and (2R,3R) 5,7,3',4'-tetramethoxyflavan-3-ol (epicatechin) 9 with a good leaving group, such as a tosyl
or mesyl group, has been hypothesized to synthesize a range of flavonoids and optically active substituted flav-3-enes.
Besides these functional groups, preactivated magnetic beads with different functional groups are available such as tosyl
or epoxy groups [78, 83].
groups on the beads were blocked with Buffer C (500 [micro]L; 4 h, 37[degrees]C), followed by washing with Buffer B (500 [micro]L; 5 min, 4[degrees]C).
Thymine, adenine, citric acid, potassium hydroxide, dichloromethane, diethyl ether, triethylamine, [alpha]-cyclodextrin, dimethylformamide, tosyl
chloride, and fluorescein were purchased from Merck (Germany).
To the suspension solution of 3 (0.3 g, 0.58 mmol) in DCM (15 mL) and [NEt.sub.3] (5mL), tosyl
chloride (0.443 g, 2.32 mmol) was slowly added.