tissue culture


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culture

 [kul´cher]
1. the propagation of microorganisms or of living tissue cells in special media conducive to their growth.
2. to induce such propagation.
3. the product of such propagation.
4. the shared values, beliefs, and practices of a particular group of people, which are transmitted from one generation to the next and are identified as patterns that guide the thinking and action of the group members. adj., adj cul´tural.
cell culture the maintenance or growth of animal cells in vitro, or a culture of such cells.
blood culture microbiologic examination of a blood sample to check for presence of microorganisms.
continuous flow culture the cultivation of bacteria in a continuous flow of fresh medium to maintain bacterial growth in logarithmic phase.
enrichment culture one grown on a medium, usually liquid, that has been supplemented to encourage the growth of a given type of organism.
hanging-drop culture a culture in which the material to be cultivated is inoculated into a drop of fluid attached to a coverglass inverted over a hollow slide.
primary culture a cell or tissue culture made by direct transfer from a natural source to an artificial medium.
selective culture one grown on a medium, usually solid, that has been supplemented to encourage the growth of a single species of microorganism. It may also include substances that inhibit the growth of other species.
shake culture a culture made by inoculating warm liquid agar culture medium in a tube and shaking to distribute contents evenly. Incubation of the resolidified culture allows the development of separated colonies; especially adaptable to obligate anaerobes.
slant culture one made on the surface of solidified medium in a tube which has been tilted to provide a greater surface area for growth.
culture-specific syndrome folk illnesses that are unique to a particular culture or geographical area. Each illness has a cluster of symptoms, signs, and behavioral changes that are recognized by members of the culture; usually, they also have a range of symbolic meanings and culturally agreed-upon treatments. Anorexia nervosa and Type A behavior pattern are examples of syndromes specific to industrialized cultures.
stab culture a culture into which the organisms are introduced by thrusting a needle deep into the medium.
streak culture a culture in which the surface of a solid medium is inoculated by drawing across it, in a zig-zag fashion, a wire inoculating loop carrying the inoculum.
suspension culture a culture in which cells multiply while suspended in a suitable medium.
tissue culture the maintaining or growing of tissue, organ primordia, or the whole or part of an organ in vitro so as to preserve its architecture and function.
type culture a culture of a species of microorganism usually maintained in a central collection of type cultures.

tis·sue cul·ture

the maintenance of live tissue after removal from the body, by placing in a vessel with a sterile nutritive medium.

tissue culture

n.
1. The technique or process of maintaining or cultivating cells or tissues derived from a living organism in a culture medium.
2. A culture of cells or tissue grown by this technique or process.

tis·sue cul·ture

(tish'ū kŭl'chŭr)
The maintenance of live tissue after removal from the body, by placing in a vessel with a sterile nutritive medium.

tissue culture

The artificial growth of sheets of human tissue in the laboratory. Tumour cells are readily cultured and some appear to be immortal. These are widely used for laboratory purposes. Normal skin cells (keratinocytes) can be cultured and used for grafting in the same person. Three-layered arteries have been grown as have sheets of urethral endothelium for purposes of urethral reconstitution in hypospadias. It has even been possible to grow a new ear around a mould of polymer mesh.

tissue culture

a technique in which individual cells grow and divide in a bath of sterile, nutritive fluid which often contains hormones and growth substances. The method is used extensively in biological laboratories, for example in cancer research (see HELA CELLS), plant breeding and routine analysis of chromosome KARYOTYPES. see AMNIOCENTESIS.
References in periodicals archive ?
Plant tissue culture is known internationally as micropropagation and is the multiplication of plants under sterile conditions with some sort of nutrient solution or medium.
'When we do tissue culture, we aim for no contaminations because if there are, the plants won't grow and propagate, which is why we have to get rid of microbial contaminations in the lab to successfully grow the plant tissues,' she said.
Under tissue culture conditions the plant genome is under stress and therefore undergoes restructuration for adaptation to the in vitro conditions (McClintock, 1984).
And Tissue Culture are hoping music lovers will do just that when they play as part of a stellar line-up of local acts at Tiny Lights' Nothing Like Christmas 2 all-day show at the Cluny 2 on Saturday, December 8.
This OpTmizer CTS T-Cell Expansion Tissue Culture Medium is intended for human ex vivo (outside the body) tissue and cell culture processing application, added the company.
According to a recent study, COPAXONE(R) stimulates T-cells to produce the neuroprotection factor BDNF (brain-derived neurotrophic factor) in tissue culture using T-cells from a COPAXONE(R) patient.
Findings from a tissue culture model of head and neck cancer suggest that gene therapy may be an effective treatment strategy.
These germplasms resulted from efforts to recover adapted creeping bentgrass plants through the use of tissue culture, ecotype collection, and laboratory and field evaluations.
If they can identify the genetic trait that controls dwarfing, then it may be possible to transfer that trait into commercial varieties that can be propagated with tissue culture.
But sometimes things that happen in tissue culture are just phenomena of the moment.
Step 3: Add just two or three drops of supernatant solution to the tissue culture vials.
Tenders are invited forthe selection of tissue culture service provider (tcsp) for the work of raising & hardening of dendrocalamus stocksii, melia dubia, bambusa vulgaris, bambusa balcooa, dendrocalamus strictus, tectona grandis& santalum album at tissue culture laboratory, rajpipla for silviculture and forest utilization office, rajpipla.