This will enable more reliable in vivo imaging of labelled cells, labelling of subcellular structures for time-lapse microscopy
and intracellular biosensing.
In the UA Systematic Bioengineering Laboratory, which Wong directs, researchers used a combination of single-cell gene expression analysis, computational modeling and time-lapse microscopy
to track leader cell formation and behavior in vitro in human breast cancer cells and in vivo in mice epithelial cells under a confocal microscope.
The method uses time-lapse microscopy
to monitor individual yeast cells undergoing a small number of divisions to form microcolonies and can measure the lag times and growth rates of as many as 80,000 individual microcolonies in a single 24-hour experiment, opening up a powerful new high-throughput tool to study the complex interplay between cell growth, division and metabolism under environmental conditions that are likely to be ecologically relevant but had previously been difficult to study in the laboratory.
However, time-lapse microscopy
of the triple mutant, which was overproducing FtsZ, revealed that branches arose from abnormal septation events.
In stunning color images using time-lapse microscopy
, scientists at the University of Illinois at Chicago for the first time have captured the very earliest stages of HIV infection in living cells.
We plan to examine the formation and function of the visual system in zebrafish larvae using in vivo time-lapse microscopy
and state-of-the-art connectomic and optogenetic approaches to monitor and perturb neuronal activity.