Using so-called time-lapse microscopy
, researchers can observe individual cells at very high time resolutions and, using fluorescent labeling, they can recognize precisely which of these proteins appear in the cell.
For time-lapse microscopy
, a gel volume of 20, wl was seeded in [mu]-slides ([mu]-Slide Angiogenesis, Ibidi); for histology and gene expression analysis, 120 [micro]l gels were prepared in 96-well plates.
With the time-lapse microscopy
, we can observe events in early embryo development in vitro.
In the UA Systematic Bioengineering Laboratory, which Wong directs, researchers used a combination of single-cell gene expression analysis, computational modeling and time-lapse microscopy
to track leader cell formation and behavior in vitro in human breast cancer cells and in vivo in mice epithelial cells under a confocal microscope.
Long-term time-lapse microscopy
analysis was performed on the culture under nonpermissive condition from day 0 to day 14 to follow the process of differentiation.
The method uses time-lapse microscopy
to monitor individual yeast cells undergoing a small number of divisions to form microcolonies and can measure the lag times and growth rates of as many as 80,000 individual microcolonies in a single 24-hour experiment, opening up a powerful new high-throughput tool to study the complex interplay between cell growth, division and metabolism under environmental conditions that are likely to be ecologically relevant but had previously been difficult to study in the laboratory.
However, time-lapse microscopy
of the triple mutant, which was overproducing FtsZ, revealed that branches arose from abnormal septation events.
In stunning color images using time-lapse microscopy
, scientists at the University of Illinois at Chicago for the first time have captured the very earliest stages of HIV infection in living cells.
At 22 hours post fertilization (hpf), injected embryos were prepared for time-lapse microscopy
. Zebrafish were anesthetized with MS222 (to inhibit spontaneous movements), treated with 0.2 mM 1-phenyl-2-thiourea (to block pigmentation), and embedded in 1.5% agarose (to immobilize the embryo).
The Barbaric research combined the use of time-lapse microscopy
, single-cell tracking and mathematical modeling to characterize bottlenecks affecting the survival of normal human embryonic stem cells and compared them with adapted cells.