In addition, glycation has detrimental effects on albumin structure, including oxidation of its free thiols
to disulfide linkages or thiol
radicals (21) and refolding of globular albumin into p-amyloid fibrils structure (p aggregates).
A standard curve was prepared from varying concentrations of cysteine to calculate the other non-protein thiol
contents in samples.
In short, disulphide bonds were first reduced to form-free functional thiol
groups with sodium borohydride.
This plasma and saliva was applied for measuring the total antioxidant capacity (TAC), total thiol
molecules (TTM) and catalase activity (CAT) in the laboratory.
In this study, the modified Ellman reagent was used to quantify the total thiol
content of the sample.
The activities of BuChE, AChE, Cu-Zn-SOD, GSH-Px, and CAT and the plasma level of CRP, TAC and thiol
proteins before and after IUI in patients are given in table 1.
Further investigations by our research group revealed that a better approach to obtain epoxy/thiol
-ene systems was to anionically polymerize the epoxy resin with allyl-functionalized tertiary amines as curing agents in conjunction with a multifunctional thiol
and a radical photoinitiator [32, 33].
The small thiols
GSH and Cys are responsible for Asc-independent reduction of Cr(VI) in cells (Zhitkovich 2005).
Two different formulas of organic nutrients have been tested on Colombard juice, which is very rich in thiol
The total thiol
concentration in the serum was determined from the standard curve using molar exctinction coefficient 1600 [M.
60] (8) (where the R group is thiol
ligand attached) have previously been prepared with atomic precision using the Brust-Schriffin method.
Glutathione is an abundant and ubiquitous low-molecular-weight thiol
that may play a role in many cellular processes, including protection against the deleterious effects of reactive oxygen species .