thioglycolate


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thi·o·gly·co·late

, thioglycollate (thī'ō-glī'kō-lāt),
A salt or ester of thioglycolic acid; frequently used in bacterial media to reduce their oxygen content so as to create favorable conditions for the growth of anaerobes; the thioglycolate will also inactivate any mercurial that might be carried over with the inoculum.
References in periodicals archive ?
For their propagation, 1000 mL Erlenmeyer flasks containing 600 mL of thioglycolate broth (8 g/L) were used.
In brief, after intraperitoneal injection of thioglycolate broth, the peritoneal cells were isolated.
injection of 1 mL of 3% thioglycolate. Animals were killed under C[O.sub.2] atmosphere, and cells were harvested by washing peritoneal cavities with 3 mL of PBS, pH 7.2, containing 10 IU/ mL heparin.
Two cones were removed from each of the 30 boxes using cotton pliers and transferred immediately to a tube containing 15 ml of fluid thioglycolate medium (fluid thioglycolate medium, Merck, Darmstadt, Germany)
At the laboratory the samples were transferred to test tubes containing thioglycolate broth, a highly nutritious medium for culturing a variety of microorganisms, and were incubated in a bacteriological incubator at 37[degrees] C for 24 hours (Oplustil, 2004).
Specimen was transported in 3 separate containers: 1 mL inoculated into a selective Todd-Hewitt enrichment media to culture for GBS in a glass bottle; 1 mL inoculated into thioglycolate enrichment media to culture for facultative anaerobes in a glass bottle; 3 mL in a sterile plastic container.
WT, SIRT2KO, and SIRT2KI mice (n = 8 each) were injected with sodium thioglycolate medium intraperitoneally, as per literature [25].
However, these aseptic cases may not have underwent appropriate culture evaluation, as a recent case series of 2 patients demonstrated an infection with Propionibacterium acnes when cultured anaerobically for 10 days on thioglycolate broth [43].
Archived bacterial samples were thawed and inoculated into sodium thioglycolate broth using sterile inoculating loop, incubated overnight at 37[degrees]C, growth detected by observing turbidity.