Enzymatic hydrolysis of these compounds by membrane-bound
thioglucosidase produces many compounds including isothiocyanates, thiocyanates, epinitriles, nitriles, and glucose.
All GSL containing plants also produce a hydrolytic enzyme (
thioglucosidase hydrolase) commonly known as myrosinase, which is physically separated from the GSLs in the intact plant tissue [7].
Total glucosinolates in Raya seeds were determined by indirect method involving hydrolysis of glucosinolate by
thioglucosidase (EC 3.2.3.1) and release of D glucose molecules (Wetter and Youngs, 1976).
