Because of the low biological concentrations of these analytes, most methods employ precolumn or postcolumn oxidation of thiamines followed by fluorescent detection of thiochrome
The oxidation of thiamine to thiochrome
was quantitatively measured by using fluorescence spectroscopy and atomic absorption spectroscopy techniques.
In October 1938, six months after I had joined Kraft, he asked me to use the recently published Jansen thiochrome
method to determine the vitamin B 1 (thiamin) in Vegemite.
The chromatographic profile of a blank injection prepared by omitting the thiochrome
reaction suggested a lack of interference in sample extracts (Fig.
18 Thiamine chloride and thiamine monophosphate (TMP) in the plasma and urine were determined by high-performance liquid chromatography (HPLC) with fluorimetric detection and precolumn derivatisation to thiochromes