6 ml of distilled water, 1 ml of TCA (trichloroacetic 10%) and 1 ml of thiobarbituric acid
The samples were drawn on 1st, 5th, 10th, 15th, and 20th day of storage for evaluation of thiobarbituric acid
reacting substances (TBARS) values, pH, water activity ([a.
2 mmol) with thiobarbituric acid
(1 mmol) and Malononitrile (1 mmol) was investigated in the presence of various catalysts (1 mmol).
(TBA; measured in milligram malonaldehyde equivalents per kilogram muscle) and TVB-N (measured in milligram N/100 g muscle) were determined according to the methods described by Botsoglou et al.
Yagi, Assay for lipid peroxidase in animal tissues by thiobarbituric acid
reaction, Anal Biochem, 95: 3518 (1979).
The thiobarbituric acid
reaction and the autoxidations of polyunsaturated fatty acid methyl esters.
The oxidant/antioxidant state was evaluated by measuring erythrocyte catalase (eCAT) and paraoxonase (PON1) activities, levels of thiobarbituric acid
(e-TBARS), serum diene, total antioxidant activity (AOA), trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP) and anti-inflammatory effects were assessed by measuring tumor necrosis factor (TNF)-[alpha], Interleukin (IL-6), IL-18, IL-10 and adiponectin levels.
Finally, the volatile oil from herb that showed the highest antioxidant property was then added to rice noodle (khanom chin) to measure the color stability and the lipid oxidation by thiobarbituric acid
Specificity of the thiobarbituric acid
reaction: its use in studies of lipid peroxidation.
This is based on the measurement of thiobarbituric acid
After incubation, all the sets were assayed for lipid peroxide content by their reaction with thiobarbituric acid
as mentioned above (Okhawa, 1978).
Chemicals: Nitroblue tetrazolium (NBT), EDTA, sodium nitropruside, thiobarbituric acid
, L ascorbic acid were purchased from Qualigens, India.