For the determination of oxidative stress, the most widely acceptable thiobarbituric acid
reactive substances assay that quantifies the concentration of malondialdehyde, a product formed as a result of degradation of unstable lipid peroxides , was exercised.
Fat oxidation was evaluated by measuring thiobarbituric acid
reactive substances (TBARS) .
was added to precipitate at 90[degrees]C for one hour.
Estimation of serum Thiobarbituric acid
reacting substances (TBARS) was done following the method described by Okhawa et al.
The method used to determine the levels of thiobarbituric acid
reactive substances (TBA-RS) was described by Ohkawa, Ohishi, and Yagi (1979), with slight modifications of De Franceschi et al.
Reaction conditions affecting the relationship between thiobarbituric acid
reactivity and lipid peroxides in human plasma.
As an index of lipid peroxidation, the yielding of thiobarbituric acid
reactive substances (TBARS) during an acid-heating reaction was determined.
Determination of malonaldehyde precursor in tissues by thiobarbituric acid
Serum levels of thiobarbituric acid
reactive substances predict cardiovascular events in patients with stable coronary artery disease: a longitudinal analysis of the PREVENT study.
Neutralization of OH radicals, which were generated in a Fenton reaction, was also monitored spectrophotometrically, based on the degradation of 2-deoxy-D-ribose to malondialdehyde (MDA), whereby MDA forms a compound with thiobarbituric acid
. The ability of the extract to inhibit generated NO radicals was examined by the use of Griess reagent.
Next, 2 mL of clear filtrate was mixed with 2 mL of 0.02 M aqueous thiobarbituric acid
We measured body weight weekly and collected blood and 24-hour urine samples for thiobarbituric acid
reactive substances (TBARS), nitric oxide (NO), and glycosuria dosages.