Barbiturates and thiobarbiturates are medicinally imperative class of heterocyclic compounds as they have been used as antipyretic , anticonvulsant , antiepileptic agents , sedatives, hypnotics [2-5], herbicidal and insecticidal , fungicidal  and antiviral agents .
A convenient way of coupling two medicinally important nucleus (Cyanoactamide and Barbiturates/Thiobarbiturates) via a single pot multicomponent reaction was employed to avail fourteen new cyanoacteamide based barbiturates and thiobarbiturates.
A myriad of determinands caught his interest: magnesium, calcium, beryllium, gold, copper, iron, and zinc; phosphorous, chloride, and protein-bound iodine; creatinine, urea, bilirubin, proteins, estrogens, nucleic acids, ketosteroids, and haptoglobin; cholesterol, triglycerides, free fatty acids, and phospholipids; salicylates, barbiturates, thiobarbiturates
, methotrexate, carbon monoxide, methanol, and ethanol; alkaline phosphatase, glucose-6-phosphatase, 5-'nucleotidase, cholinesterase, and creatine kinase.
No reports have been found on the interaction of thiobarbiturates with this enzyme.
The concentration of thiopental, a thiobarbiturate, in the plasma of the first boy was 135 mg/L by an HPLC-ultraviolet detection method.