The same lipogenesis-associated factors were then evaluated after exposure of HepG2 cells to high fructose followed by the ERS inhibitor tauroursodeoxycholic acid (TUDCA) or the ERS inducer thapsigargin
. Finally, the same lipogenesis-associated factors were evaluated in HepG2 cells after XBP-1 upregulation or downregulation through cell transfection.
The following drug stock solutions were made and used immediately or stored at -20 [degrees]C until required: 805 mmol [l.sup.-1] hexamethonium in water, 400 mmol [l.sup.-1] 2-aminoethoxydiphenyl borate (2-APB) in methanol, 10 mmol [l.sup.-1] diltiazem in water, 1 mmol [l.sup.-1] thapsigargin
in dimethyl sulfoxide (DMSO), 10 mmol [l.sup.-1] N(G)-nitro- L-arginine methyl ester (L-NAME) in ASW, and 100 mmol [l.sup.-1] theophylline in water.
The effect of [[Ca.sup.2+]]i on cell adhesion capacity was investigated using RTCA after stimulating PEO-1 cells with [IP.sub.3]-independent intracellular calcium release, thapsigargin
(TG) (50 nM, 100 nM, 500 nM, 1 [micro]M, 2 [micro]M), and extracellular calcium channel blocker TN (0,5-1-5-10-20 [micro]g/mL).
or tunicamycin was used to cause ER stress in human cells, and they were found to hinder leptin-induced STAT3 phosphorylation, establishing that ER stress induces leptin resistance.
This resulted in STIM1 clustering independent of the presence of thapsigargin
(a SERCA inhibitor used to deplete SR calcium stores, eliciting STIM1 activation).
Ogino, Comparison of Contractions Produced by Carbachol, Thapsigargin
and Cyclopiazonic Acid in the Guinea-Pig Tracheal Muscle, J.
Chemistry Briefs presents some fascinating stories, including how thapsigargin
, a molecule extracted from the poisonous Mediterranean plant Thapsia garganica, has become a powerful weapon in the oncologist's arsenal.
Inspyr Therapeutics develops therapies for cancer using a novel technology platform that combines a powerful therapeutic (thapsigargin
) with a patented prodrug delivery system that targets the release of drugs within solid tumors.
Fetal bovine serum (FBS), Fluo-4 AM, Thapsigargin
, EGTA, and Lanthanum (III) Chloride were purchased from Invitrogen, Life Sciences (Carlsbad, CA, USA).
Jansen, "Maturation and secretion of rat hepatic lipase is inhibited by alpha1B-adrenergic stimulation through changes in Ca2+ homoeostasis: thapsigargin
and EGTA both mimic the effect of adrenaline," The Biochemical Journal, vol.
triggers cardiac contractile dysfunction via NADPH oxidase-mediated mitochondrial dysfunction: role of Akt dephosphorylation," Free Radical Biology and Medicine, vol.
Using a confocal imaging technique, we here provide evidence for the existence of intracellular calcium stores that respond to muscarinic activation, having sensitivity to ryanodine and thapsigargin
possibly reflecting IP3 receptor activity and the presence of ryanodine receptors and calcium ATPase pumps.