Lung parenchyma with intravascular fat embolus (hematoxylin-eosin with osmium tetroxide
overstaining, original magnification X40).
Post fixation was made in 1 % osmium tetroxide
buffered to PH 7.4 with 0.1 M phosphate buffer at 4 [degrees]C for 1-2 hours, washed again in phosphate buffer to remove excess fixative.
They were postfixed with 1% osmium tetroxide
for 1 hour, dehydrated using gradual concentrations of ethanol (25%, 75%, 95%, and 99.9%), and then dried using hexamethyldisilazane (HMDS).
Cells were fixed in 4% paraformaldehyde, postfixed in 2% osmium tetroxide
, and embedded in EPON resin for morphological studies.
Geuna, "A simple protocol for paraffin-embedded myelin sheath staining with osmium tetroxide
for light microscope observation," Microscopy Research and Technique, vol.
Other parts of the samples were fixed in 3% glutaraldehyde, postfixed in 1% osmium tetroxide
, dehydrated through serial alcohol baths, and embedded in Epon 812 (Electron Microscopy Sciences, USA).
Pellets were post fixed in 2% buffered (0.1M sodium cacodylate) osmium tetroxide
, dehydrated through a graded ethanol series, and embedded in Spurr's resin.
Osmium powder has typical characteristic smell of that of the osmium tetroxide
. However, osmium oxide is non-volatile, black and toxic in nature.
(1) Their work examined electron density profiles measured from transmission electron micrographs of porcine stratum comeum prepared using ruthenium tetroxide
. Dense band center-to-center measurements were consistent with a 5-3-5nm arrangement; correlating to a 13nm band distances in the lamellar structures separating between the corenocytes in the stratum comeum.
The samples were (1) washed for 20 min in 90 mM sodium cacodylate buffer, (2) postfixed for 30 min in 1% osmium tetroxide
(Os[O.sub.4]) with a few granules of potassium ferrocyanide ([K.sub.4]Fe[(CN).sub.6] x 3[H.sub.2]O), (3) incubated for 30 min in 1% osmium tetroxide
, and (4) incubated in 2% aqueous uranyl acetate (U[O.sub.2](C[H.sub.3]OCO) x [H.sub.2]O) for 1 hour.
For transmission electron microscopy (TEM), small pieces of the testes were fixed in 2.5% glutaraldehyde in a 0.2-M phosphate buffer (pH 7.4) for 2 h at 4[degrees]C and post-fixed in 1% osmium tetroxide
in a phosphate buffer for 2 h at 4[degrees]C.
Moreover, TEA reduces the ignition delay of hydrazine with nitrogen tetroxide
and ignites with air at temperatures as low as -40[degrees]C 10].