The viability values, according to the
tetrazolium test, were close similarity to those of the germination.
Cerebral Infarct Volume Evaluation by Triphenyl
Tetrazolium Chloride Staining
Suitability of the
tetrazolium test methodology for recently harvested and stored coffee seeds.
The respiratory burst (NBT) activity can be quantified by the Nitroblue
Tetrazolium (NBT) assay, which measures the quantity of intracellular superoxide radicals produced by leukocytes (Sahu et al.
There was significant influence of sowing dates on protein content, and germination %, seed viability (%) with
Tetrazolium test and seed vigour (%) with Accelerated agieng at both harvest and 30 days after harvest.
Determination of reducing sugars in the nanomole range with
tetrazolium blue.
2 mL of clotted blood and 1 mL of fresh EDTA anticoagulated blood were collected for serum alpha-1 AT assay and nitroblue
tetrazolium test respectively.
Next a solution of the MTT reagent--3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide (Dia-m, Russia)--prepared using the culture medium at the final concentration of 0.
Thus, the reduction reaction of the
tetrazolium salt solution occurs only in living cells and causes changes in the staining of embryo tissues, whereas dead tissues retain their original color (FRANCA NETO et al.
Tetrazolium test: Four replications of 25 seeds were soaked in water for 18 hours; then, the embryo was separated from seed coat and nutritive tissue for later immersion in 0.
1%
tetrazolium solution at 25[degrees]C for one hour (Oliveira et al.
Briefly, the yellow MTT
tetrazolium converts to purple formazan upon removal of the bromide by diaphorase flavoprotein enzyme present in metabolically active human sperm (37--39).
