The assay assesses cell viability by detecting the formazan product formed from the reduction of 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl
tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase of metabolically active cells [29].
Tetrazolium test: This test was conducted with two subsamples of 50 seeds per lot.
We found that 46% of the seeds were viable using the
Tetrazolium test.
Isolates showed variable results for
tetrazolium reduction.
The effect of the insulin solution and formulation components on cell viability was investigated using the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyl
tetrazolium bromide] test.
The generally assessed study is cell viability with examples such as biomarkers for apoptosis, cell membrane integrity with lactase dehydrogenase (LDH) assay, comet assay for genotoxicity, immunohistochemistry, and
tetrazolium reduction assays [48].
Couto et al., "The nitroblue
tetrazolium reduction test in canine leishmaniosis," Veterinary Parasitology, vol.
(13) In brief, the method depends on the formation of nitro blue
tetrazolium chloride reduction through the xanthine-xanthine oxidase system.
The culture was positive for pH 6 , it can utilize D-Glactouronic acid, L-Aspartic acid, D-Glucuronic acid, D-Serine and was found sensitive towards Troleandomycin, Lincomycin, Vancomycin, Rifamycin,
Tetrazolium violet,
Tetrazolium blue, 1% Sodium lactate, Potassium tellurite and 1% NaCl.
To analyze the type of muscle fibers, the reaction of nicotinamide adenine dinucleotide
tetrazolium reductase (NADH-TR) was utilized, according to the model proposed by Camargo Filho et al., and used by and Castoldi et al.
The
tetrazolium salt of WST-8 [2-(2-methoxy-4-nitrophenyl) -3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] was used as a metabolism indicator, as that salt penetrates into the viable cells, where it is metabolized in the mitochondria.
