The isolation and mapping of 19 tetranucleotide
microsatellite markers in the chicken.
In the present study, we chose tetranucleotide
repeat stretches that are less compound and easier to type to develop some reliable microsatellites for M.
Enrichment of tetranucleotide
microsatellite loci from invertebrate species.
3-Mb duplication at 17p12 (reciprocal deletion for hereditary neural pressure palsy); myotonic dystrophy types 1 and 2: CTG trinucleotide repeat (type 1) and CCTG tetranucleotide
repeat (type 2); facioscapulohumeral muscular dystrophy, type 1: contraction of D4Z4 repeat at 4q with permissive 4qA telomeric haplotype; and spinal muscular atrophy (95%): homozygous deletion of SMN1.
repeat polymorphism at the human tyrosine hydroxylase gene.
In Haemophilus influenzae, type III RM system DNA methyltransferase enzyme encoding gene, mod gene, mediate PV through tetranucleotide
repeat tract motifs.
peak-height ratio for a hypothetical tetranucleotide
A general DNA structure of hybrid primer is 5'-CCTGCTCTTTCATCGGYNN-3', where first 15 bases are complementary to the oligonucleotide adapter, whereas the underlined tetranucleotide
is complementary to the genome sequence at GlaI cleavage point.
The eight loci analyzed include six di-nucleotide repeats (Sauro 2A5, Sauro 2F5, Sauro 7A9, Sauro 10H2, Sauro 13B12, and Sauro 18A4), one tri-nucleotide repeat (Sauro 1G8), and one tetranucleotide
repeat (Sauro 9B10).
As far as repeat motifs of SSR loci, the dinucleotide was the most abundant repeat type, followed by hexanucleotide, trinucleotide, tetranucleotide
, and pentanucleotide (Fig.
At a protein level, this tetranucleotide
deletion results in a disruption of the amino acid reading frame that causes arginine to be replaced by phenylalanine at position 358, with a resulting premature stop codon at position 373, p.
The search in PAL_FINDER was continued until 45 dinucleotide loci and 5 tetranucleotide
loci were discovered, requiring screening of 286,240 reads and the whole database (5,874,294 reads) for dinucleotides and tetranucleotides