SSRs are short stretches of tandemly repeated di-, tri-, or tetranucleotide
motifs (Weber, 1990) that have become the marker of choice for many genetic analyses.
The repeat motif of STR 9B was composed of a tetranucleotide
, [(TTTC).sub.n], and variation gave a continuous set of 11 alleles.
Isolation and characterization of di- and tetranucleotide
microsatellite loci in geoduck clams, Panopea abrupta.
A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide
motif, and one had an imperfect and compound motif (Table 1).
Frequencies and sequence characteristics of di-, tri, and tetranucleotide
microsatellites in wheat.
(c) Di, dinucleotide; tri, trinucleotide; tetra, tetranucleotide
; and penta, pentanucleotide repeats.
Common repeat motifs included [(CA/TG).sub.n] (72.5% of all, 88.4% of dinucleotide repeats), [(GA/TC).sub.n] (8.7% of all, 10.6% of dinucleotide repeats), [(ACA).sub.n] (5.7% of all, 73.0% of trinucleotide repeats), [(ACAG/CTGT).sub.n] (3.8 % of all, 48.6% of tetranucleotide
repeats), and [(ACGC/CGTG).sub.n] (3.2% of all, 40.5% of tetranucleotide
Simple sequence repeats are short stretches of tandomly repeated di-, tri-, or tetranucleotide
motifs (Weber, 1990).
Polymorphic stretches of DNA that consist of reiterated di-, tri-, and tetranucleotide
repeats are sprinkled throughout the genomes of humans and other organisms.
Enrichment of tetranucleotide
micorsatellite loci from invertebrate species.
Development of tri- and tetranucleotide
repeat microsatellite loci in Atlantic cod (Gadus morhua).
Chromosome-specific panels of tri- and tetranucleotide
microsatellite markers for multiplex fluorescent detection and automated genotyping: evaluation of their utility in pathology.