ii) Adrenocorticotropic hormone administration: A standardised dose (1 [micro]g/kg), of ACTH (Synacthen Depot, tetracosactide
1 mg/mL, Lot S1358, Novartis, South Africa) was administered once to each of the six cows at time 0 .
The tender number includes the following medicines: Tender no: 1, Atc code: H01aa02, Generic name: Tetracosactide
, Dispensing form: Injection, Strength: 0.25 mg / ml, Unit value: 0.25 mg, Quantity in units: 10,410.
Inadequate cortisol response to the tetracosactide
(Synacthen [R]) test in non-classic congenital adrenal hyperplasia: an exception to the rule?
Early morning cortisol was subsequently found to be low at 242 nmol/L and short Synacthen test with 250 mcg of tetracosactide
did not show adequate cortisol response (Table 1).
The majority of patients underwent screening for hypopituitarism with a tetracosactide
test as well as 72-hour fast to exclude endogenous hyperinsulinaemia, as in our index patient.
The drug is a depot formulation of Synacthen (tetracosactide
), a synthetic 24 amino acid melanocortin receptor agonist.
Addition of the ACTH analog tetracosactide caused an 8-fold increase in corticosterone secretion.
Tetracosactide (Synacthen Depot, 1 mg/mL) was obtained from Ciba (V.
The synthetic ACTH analog tetracosactide was added (11 nM) to stimulate ACTH-regulated enzyme activity in the slices.
Adrenal slices exposed to tetracosactide maintained a constant rate of [sup.3]H-leucine incorporation into protein [counts per minute (CPM) per milligram protein], which remained linear for 60 hr (Figure 1A).
In slices exposed to tetracosactide, accumulation of corticosterone and 11-deoxycorticosterone in the medium increased linearly for about 48 hr.
Launched in more than 40 countries for multiple indications, Synacthen (tetracosactide
) is a synthetic 24 amino acid melanocortin receptor agonist.