Genomic DNA from hyphal colonies developed from single teliospores
were extracted by the modified SDS method described by Que et al.
This has gained grounds for changing the prior trends set for the teliospores
Spores in irregular, subglobose to elongated groups, 30-70 x 50-110 [micron]m, of single; a single teliospore
mostly subglobose of bluntly angled; (8-)10-12(-13) x (10-)13-17(-19) [micron]m; young light yellow; old orange colored.
The isolates were derived from teliospores
collected from one diseased bud in each of three diseased populations (8, 27, and 25; Table 1).
3: Lucida drawings of Puccinia chrysanthemi (A) Urediniospores showing germ pores and echinulate ornamentation (B) Teliospores
. Scale bar: A = 11 um and B = 22 um 29-33 x 47-61 um; wall verrucose, 1.5-2.5 um; apex dark brown with hyaline papilla, conical or rounded, 3-6 um; pedicel hyaline to yellowish brown, persistent, 6-8 x 26-122 um.
Entry into the bud meristem occurred between 6 and 36 h after the teliospore
(alba) showed no obvious differences in teliospore
morphology, pattern of teliospore
germination, or sporidial size and shape.
TELIA also hypophyllous, blackish-brown; scattered or grouped, rounded or elliptic, 0.3-1 mm., pulverulent; TELIOSPORES
ellipsoid or clavate, 40-60 x 22.5-27.5 um, circular at both ends or somewhat pointed above and tapered below, septa generally constricted, light brown, 2-celled, papilla brown, 5-10 x 15-22.5 um, wall thick, brown, 2-3.5 um, thick at the sides, upper cell pore is apical, lower cell pore is near the septum; pedicel elongated, persistent, hyaline, 17.5-125 x 12.5 um in size.
The microscopic observation of the flower powder showed acute hairs, spherical, echinate pollen grains, teliospores
of Puccinia malvacearum--a typical parasite of Malva sylvestris that is very common in plants in the Ciudad Universitaria and also appears in the observation made of Althaea officinalis- and parts of subepidermis and mesophyll with oxalate druses (Figure 9).
Two types are found on daylilies in the United States and Costa Rica: urediniospores and teliospores
. Because rust fungi can usually be identified by the shape of their spores, SBML researchers tried to identify the American fungus by comparing samples taken from U.S.
In each population, diploid fungal teliospores
were collected from a single flower of two different host plants.