(tak pol'i-mĕr-ās)
A heat-stable DNA polymerase recovered from the bacterium Thermus aquaticus.
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References in periodicals archive ?
Amplification of bacterial DNA for Vt2 genes was carried out in a thermal cycler (Master cycler gradient, Germany) 10 [micro]l of prepared bacterial DNA samples was added to 90 ml of PCR mixture (10 mMTris-HCl [pH 8.3], 50 mMKCl, 1.5 mM MgCl2, 0.25 mM of each deoxynucleoside triphosphate (dATP, dTTP, dGTP, dCTP) [Pharmacia Biotech Inc.], 20 PMol of primers and 1.5 U of Taqpolymerase [Boehringer Mannheim Biochemicals, Indianapolis, Ind.])
The reaction was carried out with 25[micro]l of solution containing 5[micro]l of DNA, 1.5[micro]l of each primer (5[micro]M), 0.64 dNTPs (10mM), 1.5 [micro]l Mg[Cl.sub.2] (25mM), 6.66[micro]l ultrapure sterile [H.sub.2]O (LP), 0.2[micro]l of Taqpolymerase (5U/ml), and 5[micro]l of PCR buffer (x5 Gren Go taq).