enzyme is tannin acyl hydrolase (EC 18.104.22.168) which can only hydrolyze the galloyl residues of galloyl esters of tannins and can be used in different industrial applications 4.
Extraction and purification of tannase
by reverse micelle system.
Studies on tannase
production have been done in liquid surface, submerged or solid-state fermentation (SSF) (SABU et al., 2005; MUKHERJEE; BANERJEE, 2006; BANERJEE et al., 2007; BATTESTIN; MACEDO, 2007; RODRIGUES et al., 2007; MANJIT et al., 2008; SELWAL et al., 2011).
, also known as tannin acyl hydrolase (EC 22.214.171.124) hydrolyse ester bonds and side hydrolyzable tannins such as tannic acid, gallic acid, and glucose by releasing.
Purification and some properities of yeast tannase
However, when this gallotannin, a polymeric phenol, is attacked by tannase
produced through enzymatic activity by native plants and rhizospheric microbes, toxic gallic acid is produced and released in the root zone, exacerbating the invasive Phragmites' noxiousness.
Alternatively, gallic acid can be produced by the microbial hydrolysis of tannic acid by tannase
(tannin-acylhydrolase B 126.96.36.199), an inducible enzyme, secreted by microorganisms (Pinto et al., 2001).
Their application is direct in different industries--food and feed (alpha amylase, glucose isomerase, cellulase, xylanase, pectinase, phytase), detergent (alkaline protease, cellulase), pulp and paper (xylanase, laccase, cellulase, pectinase), leather tanning (tannase
) and pharmaceuticals (penicillin acylases, cephalosporin acylases, glutaryl 7-ACA acylases and D-amino acid oxidases) and waste treatment (laccases, tannasass).
This early colonization of fungi in tannin-rich mangrove leaves may be due to the fact that the fungi like Aspergilli are capable of producing tannase
enzyme to degrade tannin (William et al.
An Aspergillus protein or enzyme known as tannase
breaks down the tannin and unlocks the gallic acid.
In an effort to identify what might be happening, the pair added either the enzyme tannase
or lemon juice to each of the brews.
Effect of culture conditions and kinetic studies on extracellular tannase
production by Lactobacillus plantarumMTCC1407.).