gondii are not necessarily indicative of a symptomatic infection, whereas detection of tachyzoite
DNA in biological fluids as cerebral, ocular, congenital or disseminating forms can characterize a symptomatic infection.
gondii parasite causes an asymptomatic infection in most hosts due to a rapid induction of the cellular immune response, which results in the control of tachyzoite
multiplication and establishment of a chronic infection (DUBEY, 2010).
Finally (and reassuringly, if your lab has invested in commercial nucleic acid extraction systems), a commercial extraction method based on the now common chaotropic lysis/silica adsorption/wash/elute approach was the clear winner, with a reported LOD of a single tachyzoite
gondii is found in three morphologically different strains which are named as tachyzoite
, bradyzoite, and sporozoite.
However, when viewing a Toxoplasma gondii tachyzoite
at higher magnification (B, D, and F), there is a marked difference.
Stage conversion between tachyzoite
and bradyzoite forms of Toxoplasma is associated with morphological and molecular biological changes, including stage-specific antigen expression and alterations to metabolism .
showed that the serum levels of IL-10 in mice were increased following DNA vaccination with recombinant chimeric tachyzoite
After the organism enters the human host, it rapidly proliferates into the tachyzoite
form and penetrates all nucleated cells within the body.
It changes into a form that repeatedly clones itself, known as a tachyzoite
Toxoplasma gondii: determinants of tachyzoite
to bradyzoite conversion.
The organism exits in nature in many forms: macro- and microgametes, the oocyte (which releases sporozoites), the tissue cyst, which contains and may release bradyzoite, and tachyzoite
The parasite was named Toxoplasma (because of the crescent shape of the tachyzoite
with the name derived from the Greek terms toxo meaning arc or bow and plasma meaning form) gondii (after the rodent) in 1909.