synthetic medium

synthetic medium

a microbial growth medium in which all components have been chemically identified and quantified.
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While classical synthetic medium formulations support viability of a wide range of cell types, it is important to realize that medium still provides an artificial environment compared to physiologic conditions.
Test on Synthetic Medium. In test performed on synthetic liquid medium (YES 5%), the OTA containment ability of five OTA- strains against five OTA+ strains was assessed (Figure 1).
Potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beefextract, and peptone were supplemented in synthetic medium with 10 g/L (w/v) concentration.
The durable, synthetic medium uses a binder for water and light resistance, helping to prevent the ink-receiving layer from peeling.
The medium could be of a biological origin such as blood serum or tissue extract, synthetic medium or a mixture of the two.
Bt isolates were cultured on synthetic medium containing 2% colloidal chitin for screening those with chitinolytic activity (Mejia-Saules et al.
The production medium was basal synthetic medium containing 20 g sodium citrate/l with 40 g glycerol/l as reported in literature [2].
The inoculum preparation involved the following steps: (i) inoculation of 100.0 mL of synthetic medium (glucose, 10.0 g [L.sup.-1]; N[H.sub.4]N[O.sub.3], 2.0 g [L.sup.-1]; [K.sub.2]HP[O.sub.4], 1.0 g [L.sup.-1]; Na[H.sub.2]P[O.sub.4] x [H.sub.2]O, 0.4 g [L.sup.-1]; MgS[O.sub.4] x 7[H.sub.2]O, 0.5 g [L.sup.-1]; yeast extract, 2.0 g [L.sup.-1]; pH 6.5) with 25 mycelium discs (0 0.5 cm) from 7-day old MA culture; (ii) incubation at room temperature (22 [+ or -] 2[degrees]C), on a rotary shaker (100 rpm), for 7 days; (iii) washing of the resultant biomass (3 times) with sterile distilled water (d[H.sub.2]O); and (iv) biomass homogenization with 100.0 mL of sterile d[H.sub.2]O in a laboratory blender.
In the case of synthetic medium the maximum antibiotic activity was achieved at initial pH 8 (Figure 6), at temperature 34-37[degrees]C (Figure 7), 0% NaCl (w/v) (Figure 8), and 48 h incubation time (Figure 9).
[21.] Farrang SA, El-Gazor FE and EH Marth Use of the lactoperoxidase system to inactivate Escherichia coli 0157:H7 in a semi synthetic medium and in raw milk.
The present study is focused on the optimization of different parameters for the production of bacitracin using synthetic medium by Bacillus licheniformis UV-MN-HN-8.
Synthetic medium: Citric acid, 0.5 g; [KH.sub.2][PO.sub.4], 0.1g; Mg[SO.sub.4], 0.2g; acetylacetone, 0.05 ml; Fe[Cl.sub.3], 50 mg; Zn[SO.sub.4], 1.0 mg; Mn[SO.sub.4], 0.03 mg; Cu[SO.sub.4], 1.5 mg; Co[SO.sub.4], 0.3 mg; Ni[SO.sub.4], 0.1 mg; HEPES, 7 g; distilled water, 1,000 ml; pH; 5.4