The role of endothelial nitric oxide synthase (eNOS) in normal physiology suggests that it could be a potential candidate gene for cardiovascular diseases.
Nitric oxide, synthesized in vascular endothelial cells from the abundant amino acid L-arginine by the enzyme endothelial nitric oxide synthase (eNOS), is a potent vasodilator.
As the first step in analyzing the complex biochemical choreography behind a floral scent, Pichersky and his coworkers in 1994 worked out the amino acid sequence of the enzyme linalool synthase from petals of Clarkia breweri, a purple wildflower native to California.
Now, the most interesting challenge is putting it together," says Raguso, who was a graduate student in Pichersky's lab in the early 1990s and whose work led to the discovery of the linalool synthase gene.
o]-protein inhibitor pertussis toxin, by the guanylate cyclase-specific inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, and by the nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester.
This effect is mediated by the interaction with an ncmER and involves G-proteins, nitric oxide synthase (NOS), and cGMP-dependent protein kinase (PKG).
The pellets were resuspended in homogenization buffer, and ecNOS activity in the preparation was measured by the conversion of [3H]arginine to [3H]citrulline with use a Nitric Oxide Synthase Assay Kit (Calbiochem), according to the manufacturer's instructions.
Gene structure, polymorphism and mapping of the human endothelial nitric oxide synthase gene.
Furthermore, they were able to turn a white-rooted cassava cultivar into a yellow-rooted plant that accumulates beta-carotene (provitamin A) using a transgenic approach that increased the enzyme phytoene synthase
in the root.
Perspectives series: nitric oxide and nitric oxide synthases.
Nitric oxide synthases catalyze the activation of redox cycling and bioreductive anticancer agents.
It has been suggested that the single nucleotide polymorphism (SNP) in endothelial nitric oxide synthase
(eNOS) (G894T) may affect the response of vascular endothelium to increased oxidative stress.