supernatant fluid


Also found in: Dictionary, Thesaurus, Encyclopedia.

su·per·na·tant flu·id

clear fluid that, after the settling out of an insoluble liquid or solid by the action of normal gravity or of centrifugal force, takes up the upper portion of the contents of a vessel.
References in periodicals archive ?
Bacterial cells (3 g) were sonicated at 100% amplitude for 6 min and centrifuged at 12000 rpm at 4[degrees]C for 20 min, and then the supernatant fluid was used for determination of the intracellular PLP by TLC.
At the same time the quantity of DNA in the homogenate, nuclei, supernatant fluid in the liver decreased by 26.1; 21.3 and 22%, respectively, and in the intestinal mucosa--by 12%.
The supernatant fluid was centrifuged at x200000g for 60 min at 4[degrees]C, and the deposit was resuspended with PBS and stained with 2.5% sodium phosphotungustate (pH 7.0), and then examined in a Philips CM10 transmission electron microscope.
Then tubes containing these larvae were centrifuged at 1500 rpm for 5 minutes and the supernatant fluids were collected and kept at -20[degrees]C until used..
The clear supernatant was taken out and gentamycin @200ug/ml, penicillin @1000 i.u./ml and streptomycin @ 100ug/ml were added to the supernatant fluid (Rabbani, 1997).
The clarified supernatant fluid was filtered with a 0.45-[micro]m syringe filter and injected intracerebrally into a litter of suckling mice to confirm the isolation.
It was centrifuged for 8 mins at 800 rpm and supernatant fluid was removed.
Supernatant fluid was separated and used for inoculation in cell culture.
After centrifugation, the concentration of reducing sugars in the supernatant fluid were determined by the dinitrosalicylic acid method of Miller (1959).
After centrifugation, the concentration of reducing sugars in the supernatant fluid was determined by the dinitrosalicylic acid method of Miller (1959).
Estimation of residual FB1 was accompanied by measuring OD365 in UV spectrophotometer in the presence of supernatant fluids of strain MHT6 and the mutants MHT88 after 72 h of incubation at 37C.