supernatant fluid


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su·per·na·tant flu·id

clear fluid that, after the settling out of an insoluble liquid or solid by the action of normal gravity or of centrifugal force, takes up the upper portion of the contents of a vessel.
References in periodicals archive ?
Bacterial cells (3 g) were sonicated at 100% amplitude for 6 min and centrifuged at 12000 rpm at 4[degrees]C for 20 min, and then the supernatant fluid was used for determination of the intracellular PLP by TLC.
Table 1 showed that in this group of animals there were seen decrease in the DNA and RNA contents in the homogenate, nuclear fractions, supernatant fluid of the liver and intestinal mucosa.
So, the measurement of the contents of DNA and RNA in the subcellular fractions of the liver and in the mucous layer of the intestine at repeated introduction of the pesticide Decis to the animals showed that on the 30 day of a poisoning the content of DNA was significantly reduced in the homogenate, nuclei and supernatant fluid of the liver and in the mucosa of intestine.
29 Days of Liver study Nucleus Supernatant fluid DNA 30-60 4.
The marked sharp reduction of RNA was observed in the supernatant fluid of the liver.
The protein level reduced in the supernatant fluid at all the terms of investigation from 71.
In order to understand the causes of the changes of the protein synthesis it is necessary to study metabolism of the nucleic acids and total protein in the tissues and cellular organoids including supernatant fluids in the liver and intestinal mucosa (Mostafalou, Eghbal, Nili-Ahmadabadi, Baeeri, and Abdollahi, 2011).
Taking into account the above-mentioned we tried to study changes of the nucleic acids and total protein in the different parts of the cell, that is in the nucleus and mitochondrial fractions, supernatant fluids of liver and intestinal mucosa during poisoning of the laboratory animals with pesticide Karate.
Then tubes containing these larvae were centrifuged at 1500 rpm for 5 minutes and the supernatant fluids were collected and kept at -20[degrees]C until used.
After CPE was evident, the supernatant fluid was clarified by centrifugation at 3,000 rpm for 10 min.
After centrifugation, the concentration of reducing sugars in the supernatant fluid was determined by the dinitrosalicylic acid method of Miller (1959).
Estimation of residual FB1 was accompanied by measuring OD365 in UV spectrophotometer in the presence of supernatant fluids of strain MHT6 and the mutants MHT88 after 72 h of incubation at 37C.