giganteus (Rajlakshmi & Prasad) by biochemical method and neutral, sialic acid and sulfomucin
In earlier studies, researchers focused on neutral mucin type and sulfomucin
Furthermore, endogenous substrates in the large intestine, such as sulfated polysaccharides (sulfomucins
), can be used by the microflora for [H.sub.2]S generation [12, 13].
When stained with AB 1 pH very few acini are stained showing presence of very few sulfomucins. (Figure No.
When the sections are stained with AF, it shows very low colour intensity, so the presence of trace amounts of sulfomucins is confirmed.
With AF-AB staining few acini are stained blue showing presence of sialomucins while very few are stained purple confirming the presence of very few sulfomucins. (Figure No.
In that also neutral are more and in acidic sialomucins are more than sulfomucins.
Few of the acini are negative for PAS staining which may contain enzymes or some of the sulfomucins which are PAS negative.
In normal gastrointestinal mucosa, sulfomucins
are present exclusively in the colon compared to the complete exclusion of the small intestine, where sialomucins are produced.10 However few previous reports have shown that the mucin present in the metaplastic mucosa of ileostomies is colonic type.5,6
Glickman et al (8) evaluated multilayered epithelium in samples obtained from 17 patients and found that its glandular cells expressed neutral mucins, sialomucins, and sulfomucins
at rates comparable to Barrett esophagus (88%, 100%, and 71% of cases, respectively, versus 100%, 100%, and 76%, respectively).
Since phenylhydrazine blocks specially the aldehyde produced by neutral glycoprotein, only the aldehyde formed by sialomucins and sulfomucins
remain reactive to PAS (Pearse; Bancroft & Cook).
The study of the mucinous component of the neoplasm was performed using 2 stains: (1) the AB-PAS stain, which distinguishes between acid mucins stained blue by AB and neutral mucins stained magenta by PAS; and (2) the AB-HID stain, which differentiates sulfomucins
stained brownish by HID and sialomucins stained blue by AB.