Sulfite reductase (SiR) activity was estimated by the coupled SiR/OASTL assay [24, 25] with the addition of NADPH and tungstic acid .
Sagi, "A novel in-gel assay and an improved kinetic assay for determining in vitro sulfite reductase activity in plants," Plant and Cell Physiology, vol.
Sagi, "Sulfite reductase protects plants against sulfite toxicity," Plant Physiology, vol.
A second key gene of dissimilatory sulfate reduction, dsrAB, codes for the alpha and beta sub-units of the enzyme dissimilatory sulfite reductase, which catalyzes the reduction of sulfite to sulfide (Wagner et al., 1998).
Multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes.
1998 Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration.
1:45 RECOMBINANT EXPRESSION OF SULFITE REDUCTASE FROM ARABIDOPSIS THALIANA
A major constituent of plastid nucleoids is sulfite reductase (SiR), a siroheme enzyme that also participates in the sulfur assimilation pathway.
To further delineate the roles this bifunctional protein plays in the chloroplast, T-DNA insertion mutants of Arabidopsis thaliana were screened for an insertion in the sulfite reductase gene.
2:45 IDENTIFICATION OF THE CHLOROPLAST NUCLEOID PROTEIN, DCP68, AS A SULFITE REDUCTASE
N-terminal amino acid analysis and the absorption spectrum of the purified protein suggest that DCP68 is a sulfite reductase, a siroheme enzyme involved in the assimilation of sulfur for amino acid biosynthesis.