due to reduction to ABTS by GSH and secondly assessing the sulfhydryl
capacity of GSH under different conditions as shown in Figures 3 and 4.
Trivalent As inhibits many cellular enzymes by binding with sulfhydryl
groups were assayed according to the method of Ellman (Hu et al.
The unsaturated carbonyl structure of ACR is a soft electrophile that will preferentially form Michael-type adducts with soft nucleophiles, which in biological systems are sulfhydryl
side-chains on cysteine residues.
Similarly, persons who are homozygous for apolipoprotein E4, which has no sulfhydryl
group, might have a lesser capacity for eliminating xenobiotics dependent on binding to sulfhydryl
groups, such as heavy metals and some xenobiotic chemicals.
Captopril's free sulfhydryl
group produces a thiol-disulfide exchange with cystine.
Serum myeloperoxidase activity, sulfhydryl
group levels, lipid hydroperoxide levels and catalase activity were determined.
Control experiments to exclude a direct oxidant effect of PHZ (2 mM) on isolated membranes revealed no effect on band 3 sulfhydryl
groups (data not shown).
The effects of APA on gastric tissues were analyzed in terms of ulcer score (US), malondialdehyde (MDA), total proteins, non-protein sulfhydryl
(NP-SH) estimation and histological changes.
Cysteine is a sulfur containing amino acids that is unique because of its chemically very reactive sulfhydryl
complex (- SH) of glutathione directly binds to the toxic metal that has high affinity for this groups.
Similarly, intraperitoneal administration of [alpha]-santalol (100 mg/kg BW) and sandalwood oil (Ig/kg BW) for two weeks modulated parameters such as serum aminotransferases, alkaline phosphatase, bilirubin, superoxide dismutase, catalase, free sulfhydryl
, protein carbonyl, nitric oxide, liver lipid peroxide contents, and antioxidant capacity in D-galactose mediated oxidative stress induced mice.