Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase
produced in Escherichia coli BL21, Journal of Industrial Microbiology and Biotechnology, 37: 1193-201, 2010.
There were no apparent changes in liver morphology, suggesting that the observed effects on lysosomal proteases and sulfatases
were not a consequence of STZ toxicity.
As depicted in Figures 1 and 3, sulfonation of E2 forms inactive estradiol sulfate (E2S), which can be reactivated following removal of the sulfate by the cytosolic estrogen sulfatase
A Novel Common Missense Mutation G301C in the N-Acetylgalactosamine-6-sulfate Sulfatase
Gene in Mucopolysaccharidosis IVA.
The mineralisation of organic S is mediated by a combination of microbial and enzyme (sulfatase
) activities (Zhao et al.
The circumstances under which sponge cell adhesion might be influenced by these enzyme activities or their products are unclear, but may be clarified by experiments using sulfo-T inhibitors or sulfatases
, or calibrations of enzyme product responses by sponge cells after the manipulation of environmental sulfate (7).
This is especially important given that conjugated [E.sub.3] is several-fold higher in concentration than u[E.sub.3], and sulfatases
and glucuronidases that convert conjugated [E.sub.3] to u[E.sub.3] may be present in serum samples.
The sulfonation of E2 makes the compound more soluble for renal excretion as well as for the creation of inactive stores of sulfated E2 that can be desulfated by steroid sulfatases
(Coughtrie et al.
ID2S, GAL6S, and GAL4S are inhibited by the relatively high concentrations of free sulfate and phosphate in blood, and therefore the buffer contained metal cations that caused precipitation of these anions without reducing the activity of the nonsulfatases.
Because carrageenan is heavily sulfated (up to 40% by weight), bacterial sulfatases
and sulfate reductases can produce hydrogen sulfide gas or H[S.sup.-] from carrageenan.
Sulfate and phosphate ions are competitive inhibitors of sulfatases
A similar situation exists for some of the lysosomal sulfatases
in which the fluorescent substrate 4-methylumbelliferyl sulfate can be hydrolyzed by a number of sulfatases